caa a22 4500 475795733 CHVBK 20180406123723.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050017 doi (NATIONALLICENCE)springer-10.1007/s001220050017 A genetic map of an interspecific cross in Allium based on amplified fragment length polymorphism (AFLPTM) markers [Elektronische Daten] [A.W. van Heusden, J.W. van Ooijen, R. Vrielink-van Ginkel, W. H. J. Verbeek, W. A. Wietsma, C. Kik] Abstract : Segregation of 692 polymorphic AFLPTM (amplified fragment length polymorphism) fragments was determined in an F2 of the interspecific cross A. roylei x A. cepa. Two different enzyme combinations were used, PstI/MseIand EcoRI/MseI; in the latter one extra selective nucleotide was added to the MseI primer. The map based on A. cepa markers consisted of eight linkage groups with 262 markers covering 694 cM of the expected 800 cM. The map based on A. roylei markers comprised 15 linkage groups with 243 markers and had a length of 626 cM. The two maps were not integrated, and 25% of the markers remained unlinked. One of the alliinase genes and a SCAR marker linked to the disease resistance gene to downy mildew are present on this map. Of the AFLP markers, 50—80% were polymorphic between A. cepa and A. roylei; the level of polymorphic markers between different A. cepa accessions was4-8%. Springer-Verlag Berlin Heidelberg, 2000 Key words AFLP nationallicence Allium roylei nationallicence Allium cepa nationallicence Downy mildew nationallicence Genetic linkage map nationallicence Onion nationallicence van Heusden A.W. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, the Netherlands, e-mail: a.w.vanheusden@cpro.dlo.nl, Fax: +31 317 418094, NL aut van Ooijen J.W. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, the Netherlands, e-mail: a.w.vanheusden@cpro.dlo.nl, Fax: +31 317 418094, NL aut Vrielink-van Ginkel R. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, the Netherlands, e-mail: a.w.vanheusden@cpro.dlo.nl, Fax: +31 317 418094, NL aut Verbeek W. H. J. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, the Netherlands, e-mail: a.w.vanheusden@cpro.dlo.nl, Fax: +31 317 418094, NL aut Wietsma W. A. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, the Netherlands, e-mail: a.w.vanheusden@cpro.dlo.nl, Fax: +31 317 418094, NL aut Kik C. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, the Netherlands, e-mail: a.w.vanheusden@cpro.dlo.nl, Fax: +31 317 418094, NL aut https://doi.org/10.1007/s001220050017 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050017 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Heusden A.W. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, the Netherlands, e-mail: a.w.vanheusden@cpro.dlo.nl, Fax: +31 317 418094, NL aut NATIONALLICENCE 700 1- van Ooijen J.W. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, the Netherlands, e-mail: a.w.vanheusden@cpro.dlo.nl, Fax: +31 317 418094, NL aut NATIONALLICENCE 700 1- Vrielink-van Ginkel R. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, the Netherlands, e-mail: a.w.vanheusden@cpro.dlo.nl, Fax: +31 317 418094, NL aut NATIONALLICENCE 700 1- Verbeek W. H. J. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, the Netherlands, e-mail: a.w.vanheusden@cpro.dlo.nl, Fax: +31 317 418094, NL aut NATIONALLICENCE 700 1- Wietsma W. A. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, the Netherlands, e-mail: a.w.vanheusden@cpro.dlo.nl, Fax: +31 317 418094, NL aut NATIONALLICENCE 700 1- Kik C. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, the Netherlands, e-mail: a.w.vanheusden@cpro.dlo.nl, Fax: +31 317 418094, NL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer