caa a22 4500 47579575X CHVBK 20180406123723.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050015 doi (NATIONALLICENCE)springer-10.1007/s001220050015 Epistatic interaction among loci controlling the palmitic and the stearic acid levels in the seed oil of sunflower [Elektronische Daten] [B. Pérez-Vich, R. Garcés, J. M. Fernández-Martínez] Abstract : Two sunflower (Helianthus annuus L.) mutants with high concentrations of saturated fatty acids in their seed oil have been identified and studied extensively. The mutant line CAS-5 has high concentrations of palmitic acid (C16:0) (>25% compared with 7% in standard sunflower seed oil) and low-C18:0 values (3%). CAS-3 is characterized by its high levels of stearic acid (C18:0) (>22% compared with 4% in standard sunflower seed oil) and a low-C16:0 content (5%). CAS-5 also possesses elevated levels of palmitoleic acid (C16:1) (>5%), which is absent in standard sunflower seed oil. The objective of this study was to determine the relationships between the loci controlling the high-C16:0 and the high-C18:0 traits in these mutants. Plants of both mutants were reciprocally crossed. Gas chromatographic analyses of fatty acids from the seed oil of F1, F2, F3 and the BC1F1 to CAS-5 generations indicated that the loci controlling the high-C16:0 trait exerted an epistatic effect over the loci responsible for the high-C18:0 character. As a result, the phenotypic combination containing both the high-C16:0 levels of CAS-5 and the high-C18:0 levels of CAS-3 was not possible. However, phenotypes with a saturated fatty acid content of 44% (34.5% C16:0+9.5% C18:0) were identified in the F3 generation. These are the highest saturated (C16:0 and C18:0) levels reported so far in sunflower seed oil. When F3 C16:0 segregating generations in both a high- and a low-C18:0 background were compared, the high-C16:1 levels were not expressed as expected in the high-C18:0 background (CAS-3 background). In this case, the C16:1 content decreased to values below 1.5%, compared with >5% in a low-C18:0 background. As the stearoyl-ACP desaturase has been reported to catalyze the desaturation from C16:0-ACP to C16:1-ACP, these results suggested that a decrease in its activity was involved in the accumulation of C18:0 in the high-C18:0 mutant CAS-3. Springer-Verlag Berlin Heidelberg, 2000 Key words Sunflower nationallicence Helianthus annuus nationallicence High palmitic acid nationallicence High stearic acid nationallicence Epistatic interaction nationallicence Inheritance nationallicence Oil quality nationallicence Pérez-Vich B. Instituto de Agricultura Sostenible, CSIC, Apartado 4084, E-14080 Córdoba, Spain e-mail: cs9femaj@uco.es Fax: +34-957-499252, ES aut Garcés R. Instituto de la Grasa, CSIC, Apartado 1078, E-41080 Sevilla, Spain, ES aut Fernández-Martínez J. M. Instituto de Agricultura Sostenible, CSIC, Apartado 4084, E-14080 Córdoba, Spain e-mail: cs9femaj@uco.es Fax: +34-957-499252, ES aut https://doi.org/10.1007/s001220050015 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050015 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pérez-Vich B. Instituto de Agricultura Sostenible, CSIC, Apartado 4084, E-14080 Córdoba, Spain e-mail: cs9femaj@uco.es Fax: +34-957-499252, ES aut NATIONALLICENCE 700 1- Garcés R. Instituto de la Grasa, CSIC, Apartado 1078, E-41080 Sevilla, Spain, ES aut NATIONALLICENCE 700 1- Fernández-Martínez J. M. Instituto de Agricultura Sostenible, CSIC, Apartado 4084, E-14080 Córdoba, Spain e-mail: cs9femaj@uco.es Fax: +34-957-499252, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer