caa a22 4500 475795768 CHVBK 20180406123723.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050011 doi (NATIONALLICENCE)springer-10.1007/s001220050011 An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations [Elektronische Daten] [R. L. Sebastian, E. C. Howell, G. J. King, D. F. Marshall, M. J. Kearsey] Abstract : Genetical maps of molecular markers in two very different F1-derived doubled-haploid populations of Brassica oleracea are compared and the first integrated map described. The F1 crosses were: Chinese kale×calabrese (var. alboglabra×var. italica) and cauliflower×Brussels sprout (var. botrytis×var. gemmifera). Integration of the two component maps using Joinmap v.2.0 was based on 105 common loci including RFLPs, AFLPs and microsatellites. This provided an effective method of producing a high-density consensus linkage map of the B. oleracea genome. Based on 547 markers mapping to nine linkage groups, the integrated map covers a total map length of 893 cM, with an average locus interval of 2.6 cM. Comparisons back to the component linkage maps revealed similar sequences of common markers, although significant differences in recombination frequency were observed between some pairs of homologous markers. Map integration resulted in an increased locus density and effective population size, providing a stronger framework for subsequent physical mapping and for precision mapping of QTLs using substitution lines. Springer-Verlag Berlin Heidelberg, 2000 Key words Brassica oleracea nationallicence Integrated map nationallicence Molecular markers nationallicence Doubled-haploid nationallicence Comparative mapping nationallicence Sebastian R. L. Plant Genetics and Cell Biology Group, School of Biological Sciences, The University of Birmingham, Birmingham, B15 2TT, UK, GB aut Howell E. C. Plant Genetics and Cell Biology Group, School of Biological Sciences, The University of Birmingham, Birmingham, B15 2TT, UK, GB aut King G. J. Horticulture Research International, Wellesbourne, Warwickshire, CV35 9EF, UK, GB aut Marshall D. F. Plant Genetics and Cell Biology Group, School of Biological Sciences, The University of Birmingham, Birmingham, B15 2TT, UK, GB aut Kearsey M. J. Plant Genetics and Cell Biology Group, School of Biological Sciences, The University of Birmingham, Birmingham, B15 2TT, UK, GB aut https://doi.org/10.1007/s001220050011 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050011 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sebastian R. L. Plant Genetics and Cell Biology Group, School of Biological Sciences, The University of Birmingham, Birmingham, B15 2TT, UK, GB aut NATIONALLICENCE 700 1- Howell E. C. Plant Genetics and Cell Biology Group, School of Biological Sciences, The University of Birmingham, Birmingham, B15 2TT, UK, GB aut NATIONALLICENCE 700 1- King G. J. Horticulture Research International, Wellesbourne, Warwickshire, CV35 9EF, UK, GB aut NATIONALLICENCE 700 1- Marshall D. F. Plant Genetics and Cell Biology Group, School of Biological Sciences, The University of Birmingham, Birmingham, B15 2TT, UK, GB aut NATIONALLICENCE 700 1- Kearsey M. J. Plant Genetics and Cell Biology Group, School of Biological Sciences, The University of Birmingham, Birmingham, B15 2TT, UK, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer