caa a22 4500 475795806 CHVBK 20180406123724.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s001220051359 doi (NATIONALLICENCE)springer-10.1007/s001220051359 Agrobacterium-mediated engineering for sheath blight resistance of indica rice cultivars from different ecosystems [Elektronische Daten] [K. Datta, Z. Koukolíková-Nicola, N. Baisakh, N. Oliva, S.K. Datta] Abstract : A concise T-DNA element was engineered containing the rice class-I chitinase gene expressed under the control of CaMV35S and the hygromycin phosphotransferase gene (hph) as a selectable marker. The binary plasmid vector pNO1 with the T-DNA element containing these genes of interest was mobilized to Agrobacterium tumefaciens strain LBA4404 to act as an efficient donor of T-DNA in the transformation of three different indica rice cultivars from different ecosystems. Many morphologically normal, fertile transgenic plants from these rice cultivars were generated after Agrobacterium-mediated transformation using 3-week-old scutella calli as initial explants. Stable integration, inheritance and expression of the chimeric chitinase gene were demonstrated by Southern blot and Western blot analysis of the transformants. Bioassay data showed that transgenic plants can restrict the growth of the sheath blight pathogen Rhizoctonia solani. Bioassay results were correlated with the molecular analysis. Although we obtained similar results upon DNA-mediated transformation, this report shows the potential of the cost-effective, simple Agrobacterium system for genetic manipulation of rice cultivars with a pathogenesis-related (PR) gene. Springer-Verlag Berlin Heidelberg, 2000 Key words Agrobacterium nationallicence Chitinase nationallicence T-DNA nationallicence Sheath blight nationallicence Rice nationallicence Datta K. Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati City, Philippines Fax: (63-2) 8911292, 7612406 e-mail: SDATTA@CGIAR.ORG, XX aut Koukolíková-Nicola Z. Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati City, Philippines Fax: (63-2) 8911292, 7612406 e-mail: SDATTA@CGIAR.ORG, XX aut Baisakh N. Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati City, Philippines Fax: (63-2) 8911292, 7612406 e-mail: SDATTA@CGIAR.ORG, XX aut Oliva N. Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati City, Philippines Fax: (63-2) 8911292, 7612406 e-mail: SDATTA@CGIAR.ORG, XX aut Datta S.K. Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati City, Philippines Fax: (63-2) 8911292, 7612406 e-mail: SDATTA@CGIAR.ORG, XX aut https://doi.org/10.1007/s001220051359 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051359 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Datta K. Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati City, Philippines Fax: (63-2) 8911292, 7612406 e-mail: SDATTA@CGIAR.ORG, XX aut NATIONALLICENCE 700 1- Koukolíková-Nicola Z. Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati City, Philippines Fax: (63-2) 8911292, 7612406 e-mail: SDATTA@CGIAR.ORG, XX aut NATIONALLICENCE 700 1- Baisakh N. Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati City, Philippines Fax: (63-2) 8911292, 7612406 e-mail: SDATTA@CGIAR.ORG, XX aut NATIONALLICENCE 700 1- Oliva N. Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati City, Philippines Fax: (63-2) 8911292, 7612406 e-mail: SDATTA@CGIAR.ORG, XX aut NATIONALLICENCE 700 1- Datta S.K. Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati City, Philippines Fax: (63-2) 8911292, 7612406 e-mail: SDATTA@CGIAR.ORG, XX aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer