caa a22 4500 475795814 CHVBK 20180406123724.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s001220051364 doi (NATIONALLICENCE)springer-10.1007/s001220051364 Association of transgene integration sites with chromosome rearrangements in hexaploid oat [Elektronische Daten] [S. Svitashev, E. Ananiev, W. P. Pawlowski, D. A. Somers] Abstract : Transgene loci in 16 transgenic oat (Avena sativa L.) lines produced by microprojectile bombardment were characterized using phenotypic and genotypic segregation, Southern blot analysis, and fluorescence in situ hybridization (FISH). Twenty-five transgene loci were detected; 8 lines exhibited single transgene loci and 8 lines had 2 or 3 loci. Double FISH of the transgene and oat C- and A/D-genome-specific dispersed and clustered repeats showed no preferences in the distribution of transgene loci among the highly heterochromatic C genome and the A/D genomes of hexaploid oat, nor among chromosomes within the genomes. Transgene integration sites were detected at different locations along individual chromosomes, although the majority of transformants had transgenes integrated into subtelomeric and telomeric regions. Transgene integration sites exhibited different levels of structural complexity, ranging from simple integration structures of two apparently contiguous transgene copies to tightly linked clusters of multiple copies of transgenes interspersed with oat DNA. The size of the genomic interspersions observed in these transgene clusters was estimated from FISH results on prometaphase chromosomes to be megabases long, indicating that some transgene loci were significantly larger than previously determined by Southern blot analysis. Overall, 6 of the 25 transgene loci were associated with rearranged chromosomes. These results suggest that particle bombardment-mediated transgene integration may result from and cause chromosomal breakage and rearrangements. Springer-Verlag Berlin Heidelberg, 2000 Key words Genetic engineering nationallicence Oat (A. sativa L.) nationallicence Microprojectile bombardment nationallicence FISH nationallicence Transgene DNA integration nationallicence Svitashev S. Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, St.Paul, MN 55108, USA Fax: +1(612) 625-1268 e-mail: somers@biosci.cbs.umn.edu, US aut Ananiev E. Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, St.Paul, MN 55108, USA Fax: +1(612) 625-1268 e-mail: somers@biosci.cbs.umn.edu, US aut Pawlowski W. P. Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, St.Paul, MN 55108, USA Fax: +1(612) 625-1268 e-mail: somers@biosci.cbs.umn.edu, US aut Somers D. A. Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, St.Paul, MN 55108, USA Fax: +1(612) 625-1268 e-mail: somers@biosci.cbs.umn.edu, US aut https://doi.org/10.1007/s001220051364 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051364 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Svitashev S. Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, St.Paul, MN 55108, USA Fax: +1(612) 625-1268 e-mail: somers@biosci.cbs.umn.edu, US aut NATIONALLICENCE 700 1- Ananiev E. Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, St.Paul, MN 55108, USA Fax: +1(612) 625-1268 e-mail: somers@biosci.cbs.umn.edu, US aut NATIONALLICENCE 700 1- Pawlowski W. P. Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, St.Paul, MN 55108, USA Fax: +1(612) 625-1268 e-mail: somers@biosci.cbs.umn.edu, US aut NATIONALLICENCE 700 1- Somers D. A. Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, St.Paul, MN 55108, USA Fax: +1(612) 625-1268 e-mail: somers@biosci.cbs.umn.edu, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer