caa a22 4500 475795822 CHVBK 20180406123724.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s001220051358 doi (NATIONALLICENCE)springer-10.1007/s001220051358 AFLP and CAPS linkage maps of Cryptomeria japonica [Elektronische Daten] [A. M. Nikaido, T. Ujino, H. Iwata, K. Yoshimura, H. Yoshimura, Y. Suyama, M. Murai, K. Nagasaka, Y. Tsumura] Abstract : We have used two DNA marker systems, AFLP and CAPS, in a two-way pseudo-testcross strategy applied to an F1 population to construct genetic linkage maps of two local sugi cultivars. The AFLP markers detected about eight polymorphisms per parent per primer combination. Using 38 primer combinations, 612 AFLPs were detected in 'Haara 4' and 'Kumotooshi', of which 305 segregated in a 1:1 ratio (P>0.05). A total of 91 markers (83 AFLP and 8 CAPS) in 'Haara 4' and 132 (123 AFLP and 9 CAPS) in 'Kumotooshi' were distributed among 19 and 23 linkage groups, respectively, each of which included 2-17 markers. Maps of 'Haara 4' and 'Kumotooshi' spanned 1266.1 cM and 1992.3 cM, and covered approximately 50% and 80% of the sugi genome, respectively. Sequences derived from cDNA, which were previously used to construct a sugi linkage map, were also placed on our linkage maps as CAPS markers. Where a 'two-way pseudo-testcross' is used, more than half of the sugi CAPS developed can be used to construct linkage maps for each parental family. The saturation of mapped markers, and the integration of several linkage maps derived from different mapping populations, is anticipated in the near future. Springer-Verlag Berlin Heidelberg, 2000 Key words Co-dominant marker nationallicence Dominant marker nationallicence Linkage map nationallicence Sugi nationallicence Nikaido A. M. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut Ujino T. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut Iwata H. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut Yoshimura K. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut Yoshimura H. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut Suyama Y. Department of Biodiversity Science, Tohoku University, Kawatabi, Naruko, Miyagi 989-6711, Japan, JP aut Murai M. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut Nagasaka K. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut Tsumura Y. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut https://doi.org/10.1007/s001220051358 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051358 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nikaido A. M. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut NATIONALLICENCE 700 1- Ujino T. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut NATIONALLICENCE 700 1- Iwata H. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut NATIONALLICENCE 700 1- Yoshimura K. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut NATIONALLICENCE 700 1- Yoshimura H. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut NATIONALLICENCE 700 1- Suyama Y. Department of Biodiversity Science, Tohoku University, Kawatabi, Naruko, Miyagi 989-6711, Japan, JP aut NATIONALLICENCE 700 1- Murai M. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut NATIONALLICENCE 700 1- Nagasaka K. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut NATIONALLICENCE 700 1- Tsumura Y. Genetics Section, Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan e-mail: asakon@ss.ffpri.affrc.go.jp Tel.: +81-298-73-3211, 444, Fax: +81-298-73-3795, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer