caa a22 4500 475795849 CHVBK 20180406123724.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s001220051362 doi (NATIONALLICENCE)springer-10.1007/s001220051362 Microsatellite analysis of maternal half-sib families of Quercus robur, pedunculate oak: II. inferring the number of pollen donors from the offspring [Elektronische Daten] [C. Lexer, B. Heinze, S. Gerber, S. Macalka-Kampfer, H. Steinkellner, A. Kremer, J. Glössl] Abstract : We present an approach to infer the number of pollen donors directly from genotype data of open- pollinated progeny of Quercus robur (pedunculate oak), a highly outcrossing tree species. The approach is based on closely linked, highly polymorphic codominant microsatellite markers. Initially the close linkages between three previously mapped microsatellite loci were confirmed by studies of linkage disequilibrium (LD). Then an approach to track the pollen donors contributing to maternal half-sib families (open-pollinated families) was developed by analysing haplotype arrays of closely linked microsatellite markers transmitted from the fathers to the progeny. Simulated data of five linked microsatellite loci segregating in eight open-pollinated families were used to study the relationship between the number of paternal chromosomes detected by this ”haplotype approach” and the number of diploid fathers contributing to the families. The results showed that the number of diploid pollen donors can be expressed as an exponential function of the number of paternal chromosomes inferred from the progeny. The 95% confidence interval of this regression function is used to determine the minimum number of fathers contributing to a genotyped open-pollinated family of Quercus robur. Finally this open-pollinated family is used to demonstrate the resolution obtained with the ”haplotype approach”. Six independent microsatellite loci were used to study relatedness among all pairs of pollen gametes that share a haplotype of three linked markers. The results suggest that the majority of such gametes are identical by descent from the same father. The ”haplotype approach” presented here can be used to monitor the number of contributing pollen donors in commercial seedlot samples from oak or any other outcrossing tree species for which closely linked, highly polymorphic, codominant genetic markers are available. Springer-Verlag Berlin Heidelberg, 2000 Key words Oak nationallicence Quercus nationallicence Relatedness nationallicence Linkage disequilibrium nationallicence Microsatellites nationallicence Lexer C. Zentrum für Angewandte Genetik, Universität für Bodenkultur Wien, Muthgasse 18, A-1190 Vienna, Austria Fax: +43-1-36006-6392 e-mail: gloessl@edv2.boku.ac.at, AT aut Heinze B. Forstliche Bundesversuchsanstalt Wien, Hauptstraße 7, A-1140 Vienna, Austria, AT aut Gerber S. INRA, Laboratoire de Génétique et d'Amélioration des Arbres Forestiers, B.P. 45, F-33611 Gazinet Cedex, France, FR aut Macalka-Kampfer S. Zentrum für Angewandte Genetik, Universität für Bodenkultur Wien, Muthgasse 18, A-1190 Vienna, Austria Fax: +43-1-36006-6392 e-mail: gloessl@edv2.boku.ac.at, AT aut Steinkellner H. Zentrum für Angewandte Genetik, Universität für Bodenkultur Wien, Muthgasse 18, A-1190 Vienna, Austria Fax: +43-1-36006-6392 e-mail: gloessl@edv2.boku.ac.at, AT aut Kremer A. INRA, Laboratoire de Génétique et d'Amélioration des Arbres Forestiers, B.P. 45, F-33611 Gazinet Cedex, France, FR aut Glössl J. Zentrum für Angewandte Genetik, Universität für Bodenkultur Wien, Muthgasse 18, A-1190 Vienna, Austria Fax: +43-1-36006-6392 e-mail: gloessl@edv2.boku.ac.at, AT aut https://doi.org/10.1007/s001220051362 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051362 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lexer C. Zentrum für Angewandte Genetik, Universität für Bodenkultur Wien, Muthgasse 18, A-1190 Vienna, Austria Fax: +43-1-36006-6392 e-mail: gloessl@edv2.boku.ac.at, AT aut NATIONALLICENCE 700 1- Heinze B. Forstliche Bundesversuchsanstalt Wien, Hauptstraße 7, A-1140 Vienna, Austria, AT aut NATIONALLICENCE 700 1- Gerber S. INRA, Laboratoire de Génétique et d'Amélioration des Arbres Forestiers, B.P. 45, F-33611 Gazinet Cedex, France, FR aut NATIONALLICENCE 700 1- Macalka-Kampfer S. Zentrum für Angewandte Genetik, Universität für Bodenkultur Wien, Muthgasse 18, A-1190 Vienna, Austria Fax: +43-1-36006-6392 e-mail: gloessl@edv2.boku.ac.at, AT aut NATIONALLICENCE 700 1- Steinkellner H. Zentrum für Angewandte Genetik, Universität für Bodenkultur Wien, Muthgasse 18, A-1190 Vienna, Austria Fax: +43-1-36006-6392 e-mail: gloessl@edv2.boku.ac.at, AT aut NATIONALLICENCE 700 1- Kremer A. INRA, Laboratoire de Génétique et d'Amélioration des Arbres Forestiers, B.P. 45, F-33611 Gazinet Cedex, France, FR aut NATIONALLICENCE 700 1- Glössl J. Zentrum für Angewandte Genetik, Universität für Bodenkultur Wien, Muthgasse 18, A-1190 Vienna, Austria Fax: +43-1-36006-6392 e-mail: gloessl@edv2.boku.ac.at, AT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 474920674 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer