caa a22 4500 475795873 CHVBK 20180406123724.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s001220051377 doi (NATIONALLICENCE)springer-10.1007/s001220051377 Development of markers linked to Diuraphisnoxia resistance in wheat using a novel PCR-RFLP approach [Elektronische Daten] [E. Venter, A.-M. Botha] Abstracts : Through random amplified polymorphic DNA (RAPD) analysis we identified a putative marker linked to the Dn5 resistance gene. This marker was converted to a more reliable sequence-characterised-amplified regions (SCAR) marker. The initial SCAR marker amplified the correct amplification product but failed to discern between the susceptible and resistant individuals. Hence, it was utilised to sequence the internal fragment. All nested primers designed from the internal sequences were also unable to produce any polymorphism between the susceptible and resistant cultivars. Restriction digests were then performed on these fragments, and the restriction enzyme EcoRI was able to discern between the susceptible and resistant F2 individuals of the Dn5 population. This granted one marker amplified with the internal SCAR primer set OPF141083 the ability to differentiate between parental individuals carrying the Dn5 genes. This marker was tested in a segregating F2 population carrying the Dn5 resistance gene and proved able to differentiate between the segregating individuals. This marker may prove useful in marker assisted selection (MAS), although performing restriction digests may hamper the throughput of a high number of samples. Springer-Verlag Berlin Heidelberg, 2000 Key words Russian wheat aphid nationallicence Near-isogenic lines nationallicence Restriction digests nationallicence RAPD nationallicence SCAR nationallicence Venter E. Forestry and Agricultural Biotechnology Institute (FABI) and Department of Genetics, University of Pretoria, Pretoria, South Africa, 0002, ZA aut Botha A.-M Forestry and Agricultural Biotechnology Institute (FABI), Department of Genetics, and Department of Botany, University of Pretoria, Pretoria, South Africa, 0002 e-mail: ambothao@postino.up.ac.za Fax: +27-12-420-3947, ZA aut https://doi.org/10.1007/s001220051377 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051377 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Venter E. Forestry and Agricultural Biotechnology Institute (FABI) and Department of Genetics, University of Pretoria, Pretoria, South Africa, 0002, ZA aut NATIONALLICENCE 700 1- Botha A.-M Forestry and Agricultural Biotechnology Institute (FABI), Department of Genetics, and Department of Botany, University of Pretoria, Pretoria, South Africa, 0002 e-mail: ambothao@postino.up.ac.za Fax: +27-12-420-3947, ZA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer