caa a22 4500 47579589X CHVBK 20180406123724.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s001220051361 doi (NATIONALLICENCE)springer-10.1007/s001220051361 Transfer of amiprophosmethyl resistance from a Nicotiana plumbaginifolia mutant by somatic hybridisation [Elektronische Daten] [A. I. Yemets, O. P. Kundel'chuk, A. P. Smertenko, V. G. Solodushko, V. A. Rudas, Y. Y. Gleba, Y. B. Blume] Abstract : Transfer of resistance to the phosphorothioamidate herbicide, amiprophosmethyl (APM), from the β-tubulin mutant of Nicotiana plumbaginifolia to the interspecific N. plumbaginifolia (+) N. sylvestris and to the intertribal N. plumbaginifolia (+) Atropa belladonna somatic hybrids has been demonstrated. Transfer to the recipient species was accomplished by: (1) symmetric hybridisationand (2) asymmetric hybridisation using γ-irradiation of donor protoplasts. Cytogenetic analysis confirmed the hybrid origin of the hybrids obtained. It was established that most of them typically inherited no more than three donor chromosomes, although it was possible to obtain symmetric hybrids in the case of symmetric fusion. Immunofluorescent microscopy analysis has shown that protoplasts of the mutant, and of the N. plumbaginifolia (+) N. sylvestris and N. plumbaginifolia (+) A. belladonna hybrids, retained the normal structure of interphase microtubule (MT) arrays and mitotic figures after treatment with 5 µM APM, whereas MTs of protoplasts of the recipients were destroyed under these conditions. It was also shown that hybrid clones contained an altered β-tubulin isoform originating from the N. plumbaginifolia mutant. The selected hybrid clones were characterised by cross-resistance to trifluralin, a dinitroaniline herbicide with the same mode of anti-MT action. Some of the somatic hybrids which could flower were fertile. It was established that seeds of some fertile hybrids were able to germinate in the presence of 5 µM APM. The results obtained thus support the conclusion that the technique of somatic hybridisation, especially asymmetric fusion, can be used to transfer APM resistance from the N. plumbaginifolia mutant to different (related and remote) plant species of the Solanaceae, including important crops. Springer-Verlag Berlin Heidelberg, 2000 Key words Amiprophosmethyl nationallicence β-Tubulin nationallicence Nicotiana plumbaginifolia mutant nationallicence Somatic hybridisation nationallicence Transfer of resistance nationallicence Yemets A. I. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Street 148, Kiev GSP-22, 252650, Ukraine e-mail: alla@cellbio.freenet.viaduk.net, Tel./Fax: +380 44 266 7104, 2961063, UA aut Kundel'chuk O. P. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Street 148, Kiev GSP-22, 252650, Ukraine e-mail: alla@cellbio.freenet.viaduk.net, Tel./Fax: +380 44 266 7104, 2961063, UA aut Smertenko A. P. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Street 148, Kiev GSP-22, 252650, Ukraine e-mail: alla@cellbio.freenet.viaduk.net, Tel./Fax: +380 44 266 7104, 2961063, UA aut Solodushko V. G. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Street 148, Kiev GSP-22, 252650, Ukraine e-mail: alla@cellbio.freenet.viaduk.net, Tel./Fax: +380 44 266 7104, 2961063, UA aut Rudas V. A. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Street 148, Kiev GSP-22, 252650, Ukraine e-mail: alla@cellbio.freenet.viaduk.net, Tel./Fax: +380 44 266 7104, 2961063, UA aut Gleba Y. Y. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Street 148, Kiev GSP-22, 252650, Ukraine e-mail: alla@cellbio.freenet.viaduk.net, Tel./Fax: +380 44 266 7104, 2961063, UA aut Blume Y. B. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Street 148, Kiev GSP-22, 252650, Ukraine e-mail: alla@cellbio.freenet.viaduk.net, Tel./Fax: +380 44 266 7104, 2961063, UA aut https://doi.org/10.1007/s001220051361 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051361 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yemets A. I. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Street 148, Kiev GSP-22, 252650, Ukraine e-mail: alla@cellbio.freenet.viaduk.net, Tel./Fax: +380 44 266 7104, 2961063, UA aut NATIONALLICENCE 700 1- Kundel'chuk O. P. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Street 148, Kiev GSP-22, 252650, Ukraine e-mail: alla@cellbio.freenet.viaduk.net, Tel./Fax: +380 44 266 7104, 2961063, UA aut NATIONALLICENCE 700 1- Smertenko A. P. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Street 148, Kiev GSP-22, 252650, Ukraine e-mail: alla@cellbio.freenet.viaduk.net, Tel./Fax: +380 44 266 7104, 2961063, UA aut NATIONALLICENCE 700 1- Solodushko V. G. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Street 148, Kiev GSP-22, 252650, Ukraine e-mail: alla@cellbio.freenet.viaduk.net, Tel./Fax: +380 44 266 7104, 2961063, UA aut NATIONALLICENCE 700 1- Rudas V. A. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Street 148, Kiev GSP-22, 252650, Ukraine e-mail: alla@cellbio.freenet.viaduk.net, Tel./Fax: +380 44 266 7104, 2961063, UA aut NATIONALLICENCE 700 1- Gleba Y. Y. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Street 148, Kiev GSP-22, 252650, Ukraine e-mail: alla@cellbio.freenet.viaduk.net, Tel./Fax: +380 44 266 7104, 2961063, UA aut NATIONALLICENCE 700 1- Blume Y. B. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Acad. Zabolotny Street 148, Kiev GSP-22, 252650, Ukraine e-mail: alla@cellbio.freenet.viaduk.net, Tel./Fax: +380 44 266 7104, 2961063, UA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer