caa a22 4500 475795911 CHVBK 20180406123724.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s001220051376 doi (NATIONALLICENCE)springer-10.1007/s001220051376 A genetic map of the Nicotiana alata S locus that includes three pollen-expressed genes [Elektronische Daten] [J.-H. Li, N. Nass, M. Kusaba, P. N. Dodds, N. Treloar, A. E. Clarke, E. Newbigin] Abstract : The S locus of solanaceous plants includes separate genes that control the self-incompatibility phenotype of the pistil and of the pollen. The gene controlling the self-incompatibility phenotype of the pistil encodes an extracellular ribonuclease, the S-RNase. The gene(s) controlling the self-incompatibility phenotype of pollen (the pollen-S gene) has yet to be identified. As part of a long-term strategy to clone the pollen-S gene by chromosome walking, a detailed map of the region near the S locus of Nicotiana alata was generated using a total of 251 F2 plants. The map spans an interval of approximately 2.6 cM and contains five markers as well as the S-RNase gene. Two markers were detected with heterologous probes that also detect sequences linked to the S locus of Solanum tuberosum and the homologous region of the Lycopersicon genome. Three markers were identified by differential display using N. alata pollen RNA as a template. One of these markers is a pollen-expressed sequence, 48A, which detects a polymorphic marker no more than 0.5 cM from the S locus. RNA blot analysis indicates that the 48A gene is expressed primarily during pollen development after the completion of meiosis and is therefore a candidate for the pollen-S gene. The utility of these markers and the possible involvement of 48A in the molecular mechanism of self- incompatibility are discussed. Springer-Verlag Berlin Heidelberg, 2000 Key words Gametophytic self-incompatibility nationallicence S locus nationallicence Differential display nationallicence Pollen-expressed genes nationallicence Restriction fragment length polymorphisms (RFLPs) nationallicence Li J.-H Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, del Parkville, Victoria, 3010 Australia e-mail: e.newbigin@botany.unimelb.edu,au; Fax: +613-9347-1071, AU aut Nass N. Institute for Plant Biochemistry, Weinberg 3, D-06120 Halle (Saale), Germany, DE aut Kusaba M. Institute of Radiation Breeding, National Institute of Agrobiological Resources, Ministry of Agriculture, Forestry and Fisheries, Ohmiya Naka-gun, Ibaraki 319-22, Japan, JP aut Dodds P. N. Commonwealth Scientific and Industrial Research Organization, Plant Industry, GPO Box 1600, Canberra, ACT 2601, Austrialia, AT aut Treloar N. Agriculture Victoria, Attwood, Victoria 3049, Australia, AU aut Clarke A. E. Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, del Parkville, Victoria, 3010 Australia e-mail: e.newbigin@botany.unimelb.edu,au; Fax: +613-9347-1071, AU aut Newbigin E. Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, del Parkville, Victoria, 3010 Australia e-mail: e.newbigin@botany.unimelb.edu,au; Fax: +613-9347-1071, AU aut https://doi.org/10.1007/s001220051376 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051376 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li J.-H Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, del Parkville, Victoria, 3010 Australia e-mail: e.newbigin@botany.unimelb.edu,au; Fax: +613-9347-1071, AU aut NATIONALLICENCE 700 1- Nass N. Institute for Plant Biochemistry, Weinberg 3, D-06120 Halle (Saale), Germany, DE aut NATIONALLICENCE 700 1- Kusaba M. Institute of Radiation Breeding, National Institute of Agrobiological Resources, Ministry of Agriculture, Forestry and Fisheries, Ohmiya Naka-gun, Ibaraki 319-22, Japan, JP aut NATIONALLICENCE 700 1- Dodds P. N. Commonwealth Scientific and Industrial Research Organization, Plant Industry, GPO Box 1600, Canberra, ACT 2601, Austrialia, AT aut NATIONALLICENCE 700 1- Treloar N. Agriculture Victoria, Attwood, Victoria 3049, Australia, AU aut NATIONALLICENCE 700 1- Clarke A. E. Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, del Parkville, Victoria, 3010 Australia e-mail: e.newbigin@botany.unimelb.edu,au; Fax: +613-9347-1071, AU aut NATIONALLICENCE 700 1- Newbigin E. Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, del Parkville, Victoria, 3010 Australia e-mail: e.newbigin@botany.unimelb.edu,au; Fax: +613-9347-1071, AU aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer