caa a22 4500 475795938 CHVBK 20180406123724.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s001220051371 doi (NATIONALLICENCE)springer-10.1007/s001220051371 Assessment of genetic diversity within and among germplasm accessions in cultivated sorghum using microsatellite markers [Elektronische Daten] [Y. Djè, M. Heuertz, C. Lefèbvre, X. Vekemans] Abstract : Microsatellite markers are increasingly being used in crop plants to discriminate among genotypes and as tools in marker-assisted selection. Here we evaluated the use of microsatellite markers to quantify the genetic diversity within as well as among accessions sampled from the world germplasm collection of sorghum. Considerable variation was found at the five microsatellite loci analysed, with an average number of alleles per locus equal to 2.4 within accessions and 19.2 in the overall sample of 25 accessions. The collection of sorghum appeared highly structured genetically with about 70% of the total genetic diversity occurring among accessions. However, differentiation among morphologically defined races of sorghum, or among geographic origins, accounted for less than 15% of the total genetic diversity. Our results are in global agreement with those obtained previously with allozyme markers. We were also able to show that microsatellite data are useful in identifying individual accessions with a high relative contribution to the overall allelic diversity of the collection. Springer-Verlag Berlin Heidelberg, 2000 Key words Core collection nationallicence Genetic diversity nationallicence Germplasm nationallicence Microsatellites nationallicence Sorghum nationallicence SSR nationallicence Djè Y. Université Libre de Bruxelles, Laboratoire de Génétique et d'Ecologie végétales, 1850 chaussée de Wavre, B-1160 Brussels, Belgium e-mail: xvekema@ulb.ac.be, BE aut Heuertz M. CREBS Research Unit Centre de recherche public, 162a avenue de la faı¨encerie, L-1511 Luxembourg, Luxemburg, LU aut Lefèbvre C. Université Libre de Bruxelles, Laboratoire de Génétique et d'Ecologie végétales, 1850 chaussée de Wavre, B-1160 Brussels, Belgium e-mail: xvekema@ulb.ac.be, BE aut Vekemans X. Université Libre de Bruxelles, Laboratoire de Génétique et d'Ecologie végétales, 1850 chaussée de Wavre, B-1160 Brussels, Belgium e-mail: xvekema@ulb.ac.be, BE aut https://doi.org/10.1007/s001220051371 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051371 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Djè Y. Université Libre de Bruxelles, Laboratoire de Génétique et d'Ecologie végétales, 1850 chaussée de Wavre, B-1160 Brussels, Belgium e-mail: xvekema@ulb.ac.be, BE aut NATIONALLICENCE 700 1- Heuertz M. CREBS Research Unit Centre de recherche public, 162a avenue de la faı¨encerie, L-1511 Luxembourg, Luxemburg, LU aut NATIONALLICENCE 700 1- Lefèbvre C. Université Libre de Bruxelles, Laboratoire de Génétique et d'Ecologie végétales, 1850 chaussée de Wavre, B-1160 Brussels, Belgium e-mail: xvekema@ulb.ac.be, BE aut NATIONALLICENCE 700 1- Vekemans X. Université Libre de Bruxelles, Laboratoire de Génétique et d'Ecologie végétales, 1850 chaussée de Wavre, B-1160 Brussels, Belgium e-mail: xvekema@ulb.ac.be, BE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer