caa a22 4500 475795989 CHVBK 20180406123724.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s001220051369 doi (NATIONALLICENCE)springer-10.1007/s001220051369 Arnholdt-Schmitt B. Institut für Pflanzenernährung, Justus-Liebig-Universität Giessen, Südanlage 6, D-35390 Giessen, Germany e-mail: Birgit.Arnholdt-Schmitt@ernaehrung.uni-giessen.de, Present address: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 1065, 60001-970 Fortaleza, Ceará, Brasil e-mail: birgit@ufc.br, DE aut RAPD analysis: a method to investigate aspects of the reproductive biology of Hypericum perforatum L [Elektronische Daten] [B. Arnholdt-Schmitt] Abstract : Recent interest in breeding strategies for Hypericum perforatum L. requires a better understanding of the floral biology of this medicinal plant. The aim of the present study was to check, whether RAPD fingerprinting may be a useful tool for research on the mode of reproduction of this species. Progenies from three defined single plants of two accessions, as well as progenies from a random sample of seeds of a wild population, of H. perforatum were characterized by RAPD analyses using six primers. The results obtained by DNA fingerprints indicate the predominance of an identical mode of reproduction for this species, obviously due to apomixis. Nevertheless, non-identical reproduction was evident as a minor effect in H. perforatum, as could be demonstrated by significant deviations in the RAPD fingerprints of progenies from one single plant. It is concluded that RAPD fingerprint analysis is a suitable technique to discover identity or non-identity in H. perforatum populations. Therefore, RAPDs may be used in addition to cytological studies to confirm the mode of reproduction by apomixis versus self-pollination, haploid parthenogenesis or cross-fertilization. Springer-Verlag Berlin Heidelberg, 2000 Key words Apomixis nationallicence Breeding nationallicence Cross-pollination nationallicence Hypericum perforatum nationallicence RAPD fingerprints nationallicence https://doi.org/10.1007/s001220051369 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051369 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Arnholdt-Schmitt B. Institut für Pflanzenernährung, Justus-Liebig-Universität Giessen, Südanlage 6, D-35390 Giessen, Germany e-mail: Birgit.Arnholdt-Schmitt@ernaehrung.uni-giessen.de, Present address: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 1065, 60001-970 Fortaleza, Ceará, Brasil e-mail: birgit@ufc.br, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer