caa a22 4500 475796012 CHVBK 20180406123724.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s001220051383 doi (NATIONALLICENCE)springer-10.1007/s001220051383 Cytoplasmic male sterility in the olive (Olea europaea L.) [Elektronische Daten] [G. Besnard, B. Khadari, P. Villemur, A. Bervillé] Abstract : The olive tree is usually hermaphrodite but self-incompatible. In the Western Mediterranean some cultivars are totally male-sterile. Three different male-sterile phenotypes have been recognised. To infer the genetic basis of male sterility we studied its inheritance and cytoplasmic diversity in wild (oleaster) and cultivated Mediterranean olive. In the cross Olivière×Arbequina, the male-sterile trait was maternally inherited and affected all progenies. We also checked that both chloroplast and mitochondrial DNAs are maternally inherited. RFLP studies on chloroplast and mitochondrial DNAs revealed several cytotypes: two chlorotypes and four mitotypes in cultivars and oleaster (wild or feral Mediterranean olive). Furthermore, a total linkage desequilibrium between the CCK chlorotype and the MCK mitotype in cultivars and oleaster from different regions supports the fact that paternal leakage of organelles was not observed. The male sterility (ms 2) displayed by Olivière, plus six other cultivars and three oleaster was strictly associated with the CCK chlorotype and the MCK mitotype. These facts suggest that Olivière carries cytoplasmic male sterility. Male-fertile and male-sterile oleasters carrying this cytotype showed the presence of restorer alleles. This CMS might be due to a distant cross between olive taxa. The two other male-sterile phenotypes displayed by Lucques (ms 1) and Tanche (ms 3) were associated with the ME1 mitotype but we have not demonstrated CMS. Springer-Verlag Berlin Heidelberg, 2000 Key words cpDNA nationallicence Cytoplasmic male sterility nationallicence mtDNA nationallicence Olea europaea nationallicence Inheritance nationallicence RFLP nationallicence Besnard G. INRA, UR-Génétique et Amélioration des Plantes Bât 33, 2, Place Pierre Viala, F34060 Montpellier cedex 1, France e-mail: berville@ensam.inra.fr Tel.: 33 4 99 61 22 33, Fax: 33 4 67 04 54 15, FR aut Khadari B. INRA, UR-Génétique et Amélioration des Plantes Bât 33, 2, Place Pierre Viala, F34060 Montpellier cedex 1, France e-mail: berville@ensam.inra.fr Tel.: 33 4 99 61 22 33, Fax: 33 4 67 04 54 15, FR aut Villemur P. INRA, UR-Génétique et Amélioration des Plantes Bât 33, 2, Place Pierre Viala, F34060 Montpellier cedex 1, France e-mail: berville@ensam.inra.fr Tel.: 33 4 99 61 22 33, Fax: 33 4 67 04 54 15, FR aut Bervillé A. INRA, UR-Génétique et Amélioration des Plantes Bât 33, 2, Place Pierre Viala, F34060 Montpellier cedex 1, France e-mail: berville@ensam.inra.fr Tel.: 33 4 99 61 22 33, Fax: 33 4 67 04 54 15, FR aut https://doi.org/10.1007/s001220051383 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051383 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Besnard G. INRA, UR-Génétique et Amélioration des Plantes Bât 33, 2, Place Pierre Viala, F34060 Montpellier cedex 1, France e-mail: berville@ensam.inra.fr Tel.: 33 4 99 61 22 33, Fax: 33 4 67 04 54 15, FR aut NATIONALLICENCE 700 1- Khadari B. INRA, UR-Génétique et Amélioration des Plantes Bât 33, 2, Place Pierre Viala, F34060 Montpellier cedex 1, France e-mail: berville@ensam.inra.fr Tel.: 33 4 99 61 22 33, Fax: 33 4 67 04 54 15, FR aut NATIONALLICENCE 700 1- Villemur P. INRA, UR-Génétique et Amélioration des Plantes Bât 33, 2, Place Pierre Viala, F34060 Montpellier cedex 1, France e-mail: berville@ensam.inra.fr Tel.: 33 4 99 61 22 33, Fax: 33 4 67 04 54 15, FR aut NATIONALLICENCE 700 1- Bervillé A. INRA, UR-Génétique et Amélioration des Plantes Bât 33, 2, Place Pierre Viala, F34060 Montpellier cedex 1, France e-mail: berville@ensam.inra.fr Tel.: 33 4 99 61 22 33, Fax: 33 4 67 04 54 15, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer