caa a22 4500 475796047 CHVBK 20180406123724.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s001220051395 doi (NATIONALLICENCE)springer-10.1007/s001220051395 Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice [Elektronische Daten] [S. Hittalmani, A. Parco, T. V. Mew, R. S. Zeigler, N. Huang] Abstract : Three major genes (Pi1, Piz-5 and Pita) for blast resistance on chromosomes 11, 6 and 12, respectively, were fine-mapped and closely linked RFLP markers identified. New markers for Pi1 and Pita were found that were flanking the genes. The three genes were pyramided using RFLP markers. A PCR-based SAP (sequence amplified polymorphism) marker was used to identify Piz-5 in the segregating population. The plants carrying the two- and three-gene combinations that were tested for resistance to leaf blast in the Philippines and India indicated that combinations including Piz-5 have enhanced resistance than when it is present alone. The genes from the pyramided lines are at present being deployed into agronomically superior ricevarieties by marker-aided selection (MAS). Springer-Verlag Berlin Heidelberg, 2000 Key words RFLP markers nationallicence Fine-mapping nationallicence Blast resistance nationallicence Pyramiding nationallicence Marker-assisted selection nationallicence Hittalmani S. International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in, PH aut Parco A. International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in, PH aut Mew T. V. International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in, PH aut Zeigler R. S. International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in, PH aut Huang N. International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in, PH aut https://doi.org/10.1007/s001220051395 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051395 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hittalmani S. International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in, PH aut NATIONALLICENCE 700 1- Parco A. International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in, PH aut NATIONALLICENCE 700 1- Mew T. V. International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in, PH aut NATIONALLICENCE 700 1- Zeigler R. S. International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in, PH aut NATIONALLICENCE 700 1- Huang N. International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in, PH aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer