caa a22 4500 475796063 CHVBK 20180406123724.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s001220051394 doi (NATIONALLICENCE)springer-10.1007/s001220051394 Differential effects of the null alleles at the three Wx loci on the starch-pasting properties of wheat [Elektronische Daten] [E. Araki, H. Miura, S. Sawada] Abstract : The amylose/amylopectin ratio and the pasting properties of wheat starch are important in producing marketable flour products, especially Japanese noodles. To determine if null mutations at the three Wx loci confer differences in starch-pasting viscosity, we analyzed the variation associated with the null mutations in three separate sets of recombinant substitution lines of chromosomes 7A, 4A and 7D produced from crosses between Chinese Spring and three single-chromosome substitution lines carrying the null Wx alleles. Differential effects of null alleles at the three Wx loci on starch-pasting properties were revealed. With respect to chromosome 4A, the effect of the Wx-B1b allele, giving a higher peak and breakdown viscosity, was unambiguous. In addition, a QTL of minor effect was identified near the centromere on the short arm. The presence or absence of the Wx-A1 protein gave some variation in peak and breakdown viscosity, but the effects of Wx-Alb were much smaller than those of the Wx-Blb allele. Associated effects of the Wx-D1 locus were detected for the breakdown viscosity as the null Wx-D1b allele produced a higher viscosity than the wild-type Wx-D1a. While negative correlations between amylose content and breakdown viscosity were common in the three populations, the null mutations at the Wx loci produced some variation independent of amylose content. The genetic variation detected for breakdown viscosity was more evident than that for peak viscosity in all three recombinant populations. Springer-Verlag Berlin Heidelberg, 2000 Key words Triticum aestivum nationallicence Amylose content nationallicence Recombinant substitution line nationallicence Starch-pasting property nationallicence Wx gene nationallicence Araki E. Department of Crop Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan e-mail: miurahm@obihiro.ac.jp Fax: +81-155-49 5479, JP aut Miura H. Department of Crop Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan e-mail: miurahm@obihiro.ac.jp Fax: +81-155-49 5479, JP aut Sawada S. Department of Crop Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan e-mail: miurahm@obihiro.ac.jp Fax: +81-155-49 5479, JP aut https://doi.org/10.1007/s001220051394 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051394 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Araki E. Department of Crop Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan e-mail: miurahm@obihiro.ac.jp Fax: +81-155-49 5479, JP aut NATIONALLICENCE 700 1- Miura H. Department of Crop Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan e-mail: miurahm@obihiro.ac.jp Fax: +81-155-49 5479, JP aut NATIONALLICENCE 700 1- Sawada S. Department of Crop Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan e-mail: miurahm@obihiro.ac.jp Fax: +81-155-49 5479, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer