caa a22 4500 475796144 CHVBK 20180406123725.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s001220051385 doi (NATIONALLICENCE)springer-10.1007/s001220051385 Somatic cell hybridization of 'oxy' CMS Brassica juncea (AABB) with B. oleracea (CC) for correction of chlorosis and transfer of novel organelle combinations to allotetraploid brassicas [Elektronische Daten] [N. Arumugam, A. Mukhopadhyay, V. Gupta, Y. S. Sodhi, J. K. Verma, D. Pental, A. K. Pradhan] Abstract : Alloplasmic lines of cultivated Brassica species with B. oxyrrhina cytoplasm are male-sterile and suffer from severe chlorosis. We developed male-sterile lines corrected for chlorosis by fusing protoplasts of CMS B. juncea (AABB) with 'oxy' cytoplasm and normal B. oleracea (CC). A large number of male-sterile AABBCC somatic hybrids with desirable organelle combinations, i.e. chloroplasts of B. oleracea and mitochondria with recombinant genomes, were recovered. While no recombination was observed in the chloroplast genome, the mitochondrial genome showed extensive recombination that resulted in the appearance of totally novel banding patterns in some of the hybrids. Hybrids with a parental-type mitochondrial genome as well as recombinant patterns close to either of the parental types were also obtained. Using AABBCC somatic hybrids as bridging material, we transferred the desirable organelle combinations to B. juncea (AABB), B. napus (AACC), and B. carinata (BBCC). Many of these lines are now at advanced stages of backcrossing and show stable inheritance of the CMS character and do not suffer from chlorosis. Springer-Verlag Berlin Heidelberg, 2000 Key words Brassica nationallicence CMS nationallicence Somatic hybrids nationallicence Correction of chlorosis nationallicence Mitochondrial recombination nationallicence Arumugam N. Center for Genetic Manipulation of Crop Plants and Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India e-mail: pradhanakshay@hotmail.com, IN aut Mukhopadhyay A. Center for Genetic Manipulation of Crop Plants and Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India e-mail: pradhanakshay@hotmail.com, IN aut Gupta V. Center for Genetic Manipulation of Crop Plants and Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India e-mail: pradhanakshay@hotmail.com, IN aut Sodhi Y. S. Center for Genetic Manipulation of Crop Plants and Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India e-mail: pradhanakshay@hotmail.com, IN aut Verma J. K. Center for Genetic Manipulation of Crop Plants and Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India e-mail: pradhanakshay@hotmail.com, IN aut Pental D. Center for Genetic Manipulation of Crop Plants and Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India e-mail: pradhanakshay@hotmail.com, IN aut Pradhan A. K. Center for Genetic Manipulation of Crop Plants and Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India e-mail: pradhanakshay@hotmail.com, IN aut https://doi.org/10.1007/s001220051385 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051385 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arumugam N. Center for Genetic Manipulation of Crop Plants and Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India e-mail: pradhanakshay@hotmail.com, IN aut NATIONALLICENCE 700 1- Mukhopadhyay A. Center for Genetic Manipulation of Crop Plants and Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India e-mail: pradhanakshay@hotmail.com, IN aut NATIONALLICENCE 700 1- Gupta V. Center for Genetic Manipulation of Crop Plants and Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India e-mail: pradhanakshay@hotmail.com, IN aut NATIONALLICENCE 700 1- Sodhi Y. S. Center for Genetic Manipulation of Crop Plants and Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India e-mail: pradhanakshay@hotmail.com, IN aut NATIONALLICENCE 700 1- Verma J. K. Center for Genetic Manipulation of Crop Plants and Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India e-mail: pradhanakshay@hotmail.com, IN aut NATIONALLICENCE 700 1- Pental D. Center for Genetic Manipulation of Crop Plants and Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India e-mail: pradhanakshay@hotmail.com, IN aut NATIONALLICENCE 700 1- Pradhan A. K. Center for Genetic Manipulation of Crop Plants and Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India e-mail: pradhanakshay@hotmail.com, IN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer