caa a22 4500 475796152 CHVBK 20180406123725.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s001220051396 doi (NATIONALLICENCE)springer-10.1007/s001220051396 The cystathionine-γ-synthase gene involved in methionine biosynthesis is highly expressed and auxin-repressed during wild strawberry (Fragaria vesca L.) fruit ripening [Elektronische Daten] [I. Marty, C. Douat, L. Tichit, K. Jungsup, T. Leustek, G. Abagnac] Abstract : Differential screening of a cDNA library from ripe wild-strawberry (Fragaria vesca) receptacles allowed the isolation of a cDNA clone for an mRNA which accumulated to high levels in ripe strawberry receptacles. Sequence analysis of this cDNA (1940 bp) and the corresponding gene (4330 bp) indicated the presence of an ORF coding for a predicted protein of 545 amino acids (MWt 58.3 kDa) showing 91% identity with an Arabidopsis thaliana cystathionine γ-synthase (CGS), an enzyme involved in methionine biosynthesis. Up to now, CGS activity was reported only in leaves from various plants and in microorganisms. In this study, a much stronger expression was observed in receptacles from strawberries compared to other organs (leaves, flowers, etc.). Accumulation of CGS transcripts in receptacles of both wild and commercial strawberries was highly correlated with the progress of ripening. Immunoblot experiments with an A. thaliana CGS antibody demonstrated a similar profile of accumulation for CGS protein and transcripts. Southern-blot analysis of genomic DNA indicated that the CGS protein was encoded by a single gene in Fragaria species and in some climacteric fruits, e.g. peach and apricot. In addition to the ripening-related genes identified in strawberries, the treatment of receptacles (des-achenated or not) with the auxin NAA significantly reduced CGS gene expression. Springer-Verlag Berlin Heidelberg, 2000 Key words Gene regulation nationallicence Ripening-related protein nationallicence Non-climatic fruit nationallicence Marty I. INRA Avignon-Station de Technologie Produits Végétaux, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 09, France e-mail: marty@avignon.inra.fr, FR aut Douat C. INRA Avignon-Station de Technologie Produits Végétaux, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 09, France e-mail: marty@avignon.inra.fr, FR aut Tichit L. INRA Avignon-Station de Technologie Produits Végétaux, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 09, France e-mail: marty@avignon.inra.fr, FR aut Jungsup K. Biotech Center, Cook Campus, Rutgers University, New Brunswick, NJ 08903, USA, US aut Leustek T. Biotech Center, Cook Campus, Rutgers University, New Brunswick, NJ 08903, USA, US aut Abagnac G. INRA Avignon-Station de Technologie Produits Végétaux, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 09, France e-mail: marty@avignon.inra.fr, FR aut https://doi.org/10.1007/s001220051396 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051396 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marty I. INRA Avignon-Station de Technologie Produits Végétaux, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 09, France e-mail: marty@avignon.inra.fr, FR aut NATIONALLICENCE 700 1- Douat C. INRA Avignon-Station de Technologie Produits Végétaux, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 09, France e-mail: marty@avignon.inra.fr, FR aut NATIONALLICENCE 700 1- Tichit L. INRA Avignon-Station de Technologie Produits Végétaux, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 09, France e-mail: marty@avignon.inra.fr, FR aut NATIONALLICENCE 700 1- Jungsup K. Biotech Center, Cook Campus, Rutgers University, New Brunswick, NJ 08903, USA, US aut NATIONALLICENCE 700 1- Leustek T. Biotech Center, Cook Campus, Rutgers University, New Brunswick, NJ 08903, USA, US aut NATIONALLICENCE 700 1- Abagnac G. INRA Avignon-Station de Technologie Produits Végétaux, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 09, France e-mail: marty@avignon.inra.fr, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer