caa a22 4500 475796187 CHVBK 20180406123725.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s001220051431 doi (NATIONALLICENCE)springer-10.1007/s001220051431 An integrated genetic map of Populus deltoides based on amplified fragment length polymorphisms [Elektronische Daten] [R. L. Wu, Y. F. Han, J. J. Hu, J. J. Fang, L. Li, M. L. Li, Z. -B. Zeng] Abstract : Amplified fragment length polymorphism (AFLP) is an efficient molecular technique for generating a large number of DNA-based genetic markers in Populus. We have constructed an integrated genetic map for a Populus backcross population derived from two selected P. deltoides clones using AFLP markers. A traditional strategy for genetic mapping in outcrossing species, such as forest trees, is based on two-way pseudo-testcross configurations of the markers (testcross markers) heterozygous in one parent and null in the other. By using the markers segregating in both parents (intercross markers) as bridges, the two parent-specific genetic maps can be aligned. In this study, we detected a number of non-parental heteroduplex markers resulting from the PCR amplification of two DNA segments that have a high degree of homology to one another but differ in their nucleotide sequences. These heteroduplex markers detected have served as bridges to generate an integrated map which includes 19 major linkage groups equal to the Populus haploid chromosome number and 24 minor groups. The 19 major linkage groups cover a total of 2,927 cM, with an average spacing between two markers of 23. 3 cM. The map developed in this study provides a first step in producing a highly saturated linkage map of the Populus deltoides genome. Springer-Verlag Berlin Heidelberg, 2000 Key words AFLP nationallicence Heteroduplex nationallicence Intercross marker nationallicence Linkage map nationallicence Populus deltoides nationallicence Testcross marker nationallicence Wu R. L. Program in Statistical Genetics, Department of Statistics, North Carolina State University, Raleigh, NC 27695-8203 USA e-mail:rwu@statgen.ncsu.edu Fax: +1-919-515-7315 + 86, US aut Han Y. F. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091 China, CN aut Hu J. J. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091 China, CN aut Fang J. J. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091 China, CN aut Li L. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091 China, CN aut Li M. L. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091 China, CN aut Zeng Z. -B Program in Statistical Genetics, Department of Statistics, North Carolina State University, Raleigh, NC 27695-8203 USA e-mail:rwu@statgen.ncsu.edu Fax: +1-919-515-7315 + 86, US aut https://doi.org/10.1007/s001220051431 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051431 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu R. L. Program in Statistical Genetics, Department of Statistics, North Carolina State University, Raleigh, NC 27695-8203 USA e-mail:rwu@statgen.ncsu.edu Fax: +1-919-515-7315 + 86, US aut NATIONALLICENCE 700 1- Han Y. F. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091 China, CN aut NATIONALLICENCE 700 1- Hu J. J. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091 China, CN aut NATIONALLICENCE 700 1- Fang J. J. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091 China, CN aut NATIONALLICENCE 700 1- Li L. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091 China, CN aut NATIONALLICENCE 700 1- Li M. L. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091 China, CN aut NATIONALLICENCE 700 1- Zeng Z. -B Program in Statistical Genetics, Department of Statistics, North Carolina State University, Raleigh, NC 27695-8203 USA e-mail:rwu@statgen.ncsu.edu Fax: +1-919-515-7315 + 86, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer