caa a22 4500 475796195 CHVBK 20180406123725.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s001220051429 doi (NATIONALLICENCE)springer-10.1007/s001220051429 Assessment of interspecific hybridization between transgenic oilseed rape and wild radish under normal agronomic conditions [Elektronische Daten] [A. M. Chèvre, F. Eber, H. Darmency, A. Fleury, H. Picault, J. C. Letanneur, M. Renard] Abstract : In order to assess the hybridization rate between oilseed rape and wild radish under normal agronomic conditions, three 1-ha field experiments were performed. In each case, wild radish plants were transplanted at different densities in the middle, the border, or the margin of the herbicide-tolerant oilseed rape field. Among the 189084 seedlings obtained from seeds harvested on wild radish plants, only one herbicide-tolerant interspecific hybrid (RrRrAC, 2n = 37) was characterized from seeds harvested on an isolated plant growing in the margin of the field. Thus, for the wild radish total harvest, with a 95% confidence limit, the frequency of interspecific hybrids was assessed to range from 10-7 to 3.10-5. Interspecific hybrids were detected in all cases among the smallest seeds with a diameter less than 1.6 mm harvested on oilseed rape, but the highest frequency was obtained from oilseed rape close to wild radish plants growing as clusters in the border or the margin of the field. Most hybrids had the expected triploid genomic structure (ACRr, 2n = 28) except for four amphidiploids (AACCRrRr, 2n = 56) and one hybrid from a wild radish unreduced gamete (ACRrRr, 2n = 37). Among the 73847 seedlings observed on the oilseed rape total harvest, the frequency of interspecific hybrids was assessed to range from 2.10-5to 5.10-4, with a 95% confidence limit. The results are discussed with regard to the type of oilseed rape variety used and the characteristics of the interspecific hybrids. Springer-Verlag Berlin Heidelberg, 2000 Key words Transgenic oilseed rape nationallicence Wild radish nationallicence Hybridization rate nationallicence Agronomic conditions nationallicence Chèvre A. M. Station d'Amélioration des Plantes, INRA BP 35327 35653 Le Rheu cedex, France, FR aut Eber F. Station d'Amélioration des Plantes, INRA BP 35327 35653 Le Rheu cedex, France, FR aut Darmency H. Unité de Malherbologie et d'Agronomie, INRA BV 1540, 21034 Dijon, France, FR aut Fleury A. Unité de Malherbologie et d'Agronomie, INRA BV 1540, 21034 Dijon, France, FR aut Picault H. Station d'Amélioration des Plantes, INRA BP 35327 35653 Le Rheu cedex, France, FR aut Letanneur J. C. Station d'Amélioration des Plantes, INRA BP 35327 35653 Le Rheu cedex, France, FR aut Renard M. Station d'Amélioration des Plantes, INRA BP 35327 35653 Le Rheu cedex, France, FR aut https://doi.org/10.1007/s001220051429 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051429 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chèvre A. M. Station d'Amélioration des Plantes, INRA BP 35327 35653 Le Rheu cedex, France, FR aut NATIONALLICENCE 700 1- Eber F. Station d'Amélioration des Plantes, INRA BP 35327 35653 Le Rheu cedex, France, FR aut NATIONALLICENCE 700 1- Darmency H. Unité de Malherbologie et d'Agronomie, INRA BV 1540, 21034 Dijon, France, FR aut NATIONALLICENCE 700 1- Fleury A. Unité de Malherbologie et d'Agronomie, INRA BV 1540, 21034 Dijon, France, FR aut NATIONALLICENCE 700 1- Picault H. Station d'Amélioration des Plantes, INRA BP 35327 35653 Le Rheu cedex, France, FR aut NATIONALLICENCE 700 1- Letanneur J. C. Station d'Amélioration des Plantes, INRA BP 35327 35653 Le Rheu cedex, France, FR aut NATIONALLICENCE 700 1- Renard M. Station d'Amélioration des Plantes, INRA BP 35327 35653 Le Rheu cedex, France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer