caa a22 4500 475796233 CHVBK 20180406123725.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s001220051426 doi (NATIONALLICENCE)springer-10.1007/s001220051426 An assessment of genetic relationships within the genus Digitalis based on PCR-generated RAPD markers [Elektronische Daten] [S. G. Nebauer, L. del Castillo-Agudo, J. Segura] Abstract : RAPD markers were used to study inter-specific variation among six species of the genus Digitalis: D. obscura, D. lanata, D. grandiflora, D. purpurea, D. thapsi and D. dubia, and the hybrid D. excelsior (D. purpurea×D. grandiflora). A total of 91 highly reproducible bands amplified with four arbitrarily chosen decamer primers were obtained. Homology of the co-emigrating RAPD markers was tested by blot hybridisation and sequencing of selected bands. The application of a range of statistical approaches for RAPD data analysis, including distance and parsimony methods, family clustering and the analysis of molecular variance (AMOVA), indicated that these molecular markers were taxonomically informative in Digitalis. The species relationships revealed were fully consistent with those previously obtained using morphological affinities. The hybrid D. excelsior seems to have stronger affinity to the section Digitalis than to Grandiflorae. This is the first known report of the application of RAPD markers for the study of genetic relationships among species of the genus Digitalis. Springer-Verlag Berlin Heidelberg, 2000 Key words Digitalis spp nationallicence AMOVA nationallicence Genetic relationships nationallicence RAPD nationallicence Scrophulariaceae nationallicence Nebauer S. G. Departamento de Biología Vegetal, Facultad de Farmacia, Universitat de València, Av. V.A. Estellés s/n, 46100-Burjassot, València, Spain e-mail: juan.segura@uv.es Tel.: +34963864922, Fax: +34963864926, ES aut del Castillo-Agudo L. Departamento de Microbiología y Ecología, Facultad de Farmacia, Universitat de València, Spain, ES aut Segura J. Departamento de Biología Vegetal, Facultad de Farmacia, Universitat de València, Av. V.A. Estellés s/n, 46100-Burjassot, València, Spain e-mail: juan.segura@uv.es Tel.: +34963864922, Fax: +34963864926, ES aut https://doi.org/10.1007/s001220051426 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051426 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nebauer S. G. Departamento de Biología Vegetal, Facultad de Farmacia, Universitat de València, Av. V.A. Estellés s/n, 46100-Burjassot, València, Spain e-mail: juan.segura@uv.es Tel.: +34963864922, Fax: +34963864926, ES aut NATIONALLICENCE 700 1- del Castillo-Agudo L. Departamento de Microbiología y Ecología, Facultad de Farmacia, Universitat de València, Spain, ES aut NATIONALLICENCE 700 1- Segura J. Departamento de Biología Vegetal, Facultad de Farmacia, Universitat de València, Av. V.A. Estellés s/n, 46100-Burjassot, València, Spain e-mail: juan.segura@uv.es Tel.: +34963864922, Fax: +34963864926, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer