caa a22 4500 475796322 CHVBK 20180406123725.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s001220051427 doi (NATIONALLICENCE)springer-10.1007/s001220051427 Location and mapping of the powdery mildew resistance gene MlRE and detection of a resistance QTL by bulked segregant analysis (BSA) with microsatellites in wheat [Elektronische Daten] [N. Chantret, P. Sourdille, M. Röder, M. Tavaud, M. Bernard, G. Doussinault] Abstract : Powdery mildew (Blumeria graminis f. sp. tritici) is one of the most damaging diseases of wheat (Triticum aestivum). The objective of this study was to locate and map a recently identified powdery mildew resistance gene, MlRE, carried by the resistant line RE714 using microsatellites uniformly distributed among the whole genome together with a bulked segregant analysis (BSA). The bulks consisted of individuals with an extreme phenotype taken from a population of 140 F3 families issued from the cross between RE714 (resistant) and Hardi (susceptible). The population had been tested with three powdery mildew isolates at the seedling stage. Qualitative interpretation of the resistance tests located the MlRE gene on the distal part of the long arm of chromosome 6A. A subsequent quantitative interpretation of the resistance permitted us to detect another resistance factor on a linkage group assigned to chromosome 5D, which was constructed with microsatellites for which a polymorphism of intensity between bulks was observed. This quantitative trait locus (QTL) explained 16.8- 25.34% of the total variation. An interaction between both the resistant factor (MlRE and the QTL) was found for only one of the isolates tested. This study shows the advantage of making a quantitative interpretation of resistant tests and that the use of microsatellites combined with BSA is a powerful strategy to locate resistance genes in wheat. Springer-Verlag Berlin Heidelberg, 2000 Key words Triticum aestivum nationallicence Blumeria graminis f. sp. tritici nationallicence QTL mapping nationallicence Molecular markers nationallicence Disease resistance nationallicence Chantret N. I.N.R.A. Station d'Amélioration des Plantes, Domaine de la Motte, 35653 Le Rheu Cedex, France, FR aut Sourdille P. I.N.R.A. Station d'Amélioration des Plantes, Domaine de Crouelle, 234 Avenue du Brézet, 63039 Clermont-Ferrand Cedex 2, France, FR aut Röder M. Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, 06466 Gatersleben, Germany, DE aut Tavaud M. I.N.R.A. Station d'Amélioration des Plantes, Domaine de Crouelle, 234 Avenue du Brézet, 63039 Clermont-Ferrand Cedex 2, France, FR aut Bernard M. I.N.R.A. Station d'Amélioration des Plantes, Domaine de Crouelle, 234 Avenue du Brézet, 63039 Clermont-Ferrand Cedex 2, France, FR aut Doussinault G. I.N.R.A. Station d'Amélioration des Plantes, Domaine de la Motte, 35653 Le Rheu Cedex, France, FR aut https://doi.org/10.1007/s001220051427 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051427 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chantret N. I.N.R.A. Station d'Amélioration des Plantes, Domaine de la Motte, 35653 Le Rheu Cedex, France, FR aut NATIONALLICENCE 700 1- Sourdille P. I.N.R.A. Station d'Amélioration des Plantes, Domaine de Crouelle, 234 Avenue du Brézet, 63039 Clermont-Ferrand Cedex 2, France, FR aut NATIONALLICENCE 700 1- Röder M. Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, 06466 Gatersleben, Germany, DE aut NATIONALLICENCE 700 1- Tavaud M. I.N.R.A. Station d'Amélioration des Plantes, Domaine de Crouelle, 234 Avenue du Brézet, 63039 Clermont-Ferrand Cedex 2, France, FR aut NATIONALLICENCE 700 1- Bernard M. I.N.R.A. Station d'Amélioration des Plantes, Domaine de Crouelle, 234 Avenue du Brézet, 63039 Clermont-Ferrand Cedex 2, France, FR aut NATIONALLICENCE 700 1- Doussinault G. I.N.R.A. Station d'Amélioration des Plantes, Domaine de la Motte, 35653 Le Rheu Cedex, France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer