caa a22 4500 475796349 CHVBK 20180406123725.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s001220051430 doi (NATIONALLICENCE)springer-10.1007/s001220051430 Development and incorporation of microsatellite markers into the linkage map of sugar beet (Beta vulgaris spp.) [Elektronische Daten] [S. J. Rae, C. Aldam, I. Dominguez, M. Hoebrechts, S. R. Barnes, K. J. Edwards] Abstract : A set of informative simple sequence repeat markers has been identified for use in the marker-assisted breeding of Beta vulgaris. Highly enriched small insert genomic libraries were constructed, consisting of 1536 clones (with inserts of between 250-900 bp). Screening the clones with CA, CT, CAA, CATA and GATA nucleotide-repeat probes revealed positive hybridisation to over 50% of the clones. Of these 340 were sequenced. Primer pairs were designed for sequences flanking the repeats and, of these, 57 pairs revealed length polymorphism with 12 Beta accessions. Heterozygosity levels of the SSR loci ranged from 0.069 to 0.809. Heterozygosity levels were found to be similar to those detected employing RFLP probes with the same accessions. Phenetic analysis using the markers, indicated relationships in accordance with known pedigrees. Twenty three of the SSR markers were polymorphic in one or both of two F2 mapping populations, and were placed relative to a framework of RFLP probes. The markers are distributed over all nine linkage groups of sugar beet. Springer-Verlag Berlin Heidelberg, 2000 Key words Simple sequence repeats nationallicence Linkage maps nationallicence Sugar beet nationallicence Marker-assisted selection nationallicence Rae S. J. IACR-Long Ashton Research Station, Institute of Arable Crop Research, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UK e-mail: Keith-Edwards@bbsrc.ac.uk, GB aut Aldam C. IACR-Long Ashton Research Station, Institute of Arable Crop Research, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UK e-mail: Keith-Edwards@bbsrc.ac.uk, GB aut Dominguez I. ADVANTA Biotechnology Department, SES Europe N.V., Industriepark, Soldatenplein Z2 nr. 15, B-3300 Tienen, Belgium, BE aut Hoebrechts M. ADVANTA Biotechnology Department, SES Europe N.V., Industriepark, Soldatenplein Z2 nr. 15, B-3300 Tienen, Belgium, BE aut Barnes S. R. ADVANTA Biotechnology Department, SES Europe N.V., Industriepark, Soldatenplein Z2 nr. 15, B-3300 Tienen, Belgium, BE aut Edwards K. J. IACR-Long Ashton Research Station, Institute of Arable Crop Research, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UK e-mail: Keith-Edwards@bbsrc.ac.uk, GB aut https://doi.org/10.1007/s001220051430 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051430 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rae S. J. IACR-Long Ashton Research Station, Institute of Arable Crop Research, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UK e-mail: Keith-Edwards@bbsrc.ac.uk, GB aut NATIONALLICENCE 700 1- Aldam C. IACR-Long Ashton Research Station, Institute of Arable Crop Research, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UK e-mail: Keith-Edwards@bbsrc.ac.uk, GB aut NATIONALLICENCE 700 1- Dominguez I. ADVANTA Biotechnology Department, SES Europe N.V., Industriepark, Soldatenplein Z2 nr. 15, B-3300 Tienen, Belgium, BE aut NATIONALLICENCE 700 1- Hoebrechts M. ADVANTA Biotechnology Department, SES Europe N.V., Industriepark, Soldatenplein Z2 nr. 15, B-3300 Tienen, Belgium, BE aut NATIONALLICENCE 700 1- Barnes S. R. ADVANTA Biotechnology Department, SES Europe N.V., Industriepark, Soldatenplein Z2 nr. 15, B-3300 Tienen, Belgium, BE aut NATIONALLICENCE 700 1- Edwards K. J. IACR-Long Ashton Research Station, Institute of Arable Crop Research, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UK e-mail: Keith-Edwards@bbsrc.ac.uk, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer