caa a22 4500 47579639X CHVBK 20180406123725.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s001220051419 doi (NATIONALLICENCE)springer-10.1007/s001220051419 Citrus phylogeny and genetic origin of important species as investigated by molecular markers [Elektronische Daten] [E. Nicolosi, Z. N. Deng, A. Gentile, S. La Malfa, G. Continella, E. Tribulato] Abstract : Citrus phylogeny was investigated using RAPD, SCAR and cpDNA markers. The genotypes analyzed included 36 accessions belonging to Citrus together with 1 accession from each of the related genera Poncirus, Fortunella, Microcitrus and Eremocitrus. Phylogenetic analysis with 262 RAPDs and 14 SCARs indicated that Fortunella is phylogenetically close to Citrus while the other three related genera are distant from Citrus and from each other. Within Citrus, the separation into two subgenera, Citrus and Papeda, designated by Swingle, was clearly observed except for C. celebica and C. indica. Almost all the accessions belonging to subgenus Citrus fell into three clusters, each including 1 genotype that was considered to be a true species. Different phylogenetic relationships were revealed with cpDNA data. Citrus genotypes were separated into subgenera Archicitrus and Metacitrus, as proposed by Tanaka, while the division of subgenera Citrus and Papeda disappeared. C. medica and C. indica were quite distant from other citrus as well from related genera. C. ichangensis appeared to be the ancestor of the mandarin cluster, including C. tachibana. Lemon and Palestine sweet lime were clustered into the Pummelo cluster led by C. latipes. C. aurantifolia was located in the Micrantha cluster. Furthermore, genetic origin was studied on 17 cultivated citrus genotypes by the same molecular markers, and a hybrid origin was hypothesized for all the tested genotypes. The assumptions are discussed with respect to previous studies; similar results were obtained for the origin of orange and grapefruit. Hybrids of citron and sour orange were assumed for lemon, Palestine sweet lime, bergamot and Volkamer lemon, while a citron × mandarin hybrid was assumed for Rangpur lime and Rough lemon. For Mexican lime our molecular data indicated C. micrantha to be the female parent and C. medica as the male one. Springer-Verlag Berlin Heidelberg, 2000 Key words Citrus nationallicence RAPD nationallicence SCAR nationallicence cpDNA nationallicence Phylogeny nationallicence Origin nationallicence Nicolosi E. Istituto di Coltivazioni arboree, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy Fax: +39-095-356322 e-mail: nicoeli@mbox.fagr.unict.it, IT aut Deng Z. N. Istituto di Coltivazioni arboree, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy Fax: +39-095-356322 e-mail: nicoeli@mbox.fagr.unict.it, IT aut Gentile A. Istituto di Coltivazioni arboree, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy Fax: +39-095-356322 e-mail: nicoeli@mbox.fagr.unict.it, IT aut La Malfa S. Istituto di Coltivazioni arboree, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy Fax: +39-095-356322 e-mail: nicoeli@mbox.fagr.unict.it, IT aut Continella G. Istituto di Coltivazioni arboree, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy Fax: +39-095-356322 e-mail: nicoeli@mbox.fagr.unict.it, IT aut Tribulato E. Istituto di Coltivazioni arboree, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy Fax: +39-095-356322 e-mail: nicoeli@mbox.fagr.unict.it, IT aut https://doi.org/10.1007/s001220051419 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051419 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nicolosi E. Istituto di Coltivazioni arboree, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy Fax: +39-095-356322 e-mail: nicoeli@mbox.fagr.unict.it, IT aut NATIONALLICENCE 700 1- Deng Z. N. Istituto di Coltivazioni arboree, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy Fax: +39-095-356322 e-mail: nicoeli@mbox.fagr.unict.it, IT aut NATIONALLICENCE 700 1- Gentile A. Istituto di Coltivazioni arboree, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy Fax: +39-095-356322 e-mail: nicoeli@mbox.fagr.unict.it, IT aut NATIONALLICENCE 700 1- La Malfa S. Istituto di Coltivazioni arboree, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy Fax: +39-095-356322 e-mail: nicoeli@mbox.fagr.unict.it, IT aut NATIONALLICENCE 700 1- Continella G. Istituto di Coltivazioni arboree, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy Fax: +39-095-356322 e-mail: nicoeli@mbox.fagr.unict.it, IT aut NATIONALLICENCE 700 1- Tribulato E. Istituto di Coltivazioni arboree, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy Fax: +39-095-356322 e-mail: nicoeli@mbox.fagr.unict.it, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer