caa a22 4500 475796403 CHVBK 20180406123725.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s001220051353 doi (NATIONALLICENCE)springer-10.1007/s001220051353 Genetic polymorphism in low-molecular-weight glutenin genes from Triticum aestivum , variety Chinese Spring [Elektronische Daten] [M. Benmoussa, L.-P. Vézina, M. Pagé, S. Yelle, S. Laberge] Abstract : Low-molecular-weight (LMW) glutenin subunits consist mainly of two domains, one at the N- terminus which contains repeats of short amino-acid motifs, and a non-repetitive one rich in cysteine, at the C- terminal region. In previous reports, polyacrylamide-gel electrophoresis has been used to show that large size variation exists among LMW and HMW glutenin subunits, and it has been suggested that deletions and insertions within the repetitive region are responsible for these variations in length. In this study, PCR-amplification of genomic DNA (Triticum aestivum variety Chinese Spring) was used to isolate three full-length LMW glutenin genes: LMWG-MB1, LMWG-MB2 and LMWG-MB3. The deduced amino-acid sequences show a high similarity between these ORFs, and with those of other LMW glutenin genes. Comparisons indicate that LMWG-MB1 has probably lost a 12-bp fragment through deletion and that LMWG-MB1 and LMWG-MB2 have an insertion of 81 bp within the repetitive domain. The current study has shown direct evidence that insertions and/or deletions provide a mechanistic explanation for the allelic variation, and the resultant evolution, of prolamin genes. Single-base substitutions at identical sites generate stop codons in both LMWG-MB2 and LMWG-MB3 indicating that these clones are pseudogenes. Springer-Verlag Berlin Heidelberg, 2000 Key words Glutenin nationallicence Triticum aestivum nationallicence Wheat storage proteins nationallicence Benmoussa M. Centre de recherche en horticulture, Département de phytologie, FSAA, Université Laval, Québec G1K 7P4, Canada, CA aut Vézina L.-P Agriculture et Agroalimentaire Canada, 2560 boul. Hochelage, Ste-Foy Québec G1V 2J3, Canada e-mail: laberges@em.agr.ca Tel.: +1-(418) 657-7980, Fax: +1-(418) 648-2402, CA aut Pagé M. Laboratoire de cancérologie, Département de biochimie, Faculté de médecine, Université Laval, Québec G1K 7P4, Canada, CA aut Yelle S. Centre de recherche en horticulture, Département de phytologie, FSAA, Université Laval, Québec G1K 7P4, Canada, CA aut Laberge S. Agriculture et Agroalimentaire Canada, 2560 boul. Hochelage, Ste-Foy Québec G1V 2J3, Canada e-mail: laberges@em.agr.ca Tel.: +1-(418) 657-7980, Fax: +1-(418) 648-2402, CA aut https://doi.org/10.1007/s001220051353 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051353 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Benmoussa M. Centre de recherche en horticulture, Département de phytologie, FSAA, Université Laval, Québec G1K 7P4, Canada, CA aut NATIONALLICENCE 700 1- Vézina L.-P Agriculture et Agroalimentaire Canada, 2560 boul. Hochelage, Ste-Foy Québec G1V 2J3, Canada e-mail: laberges@em.agr.ca Tel.: +1-(418) 657-7980, Fax: +1-(418) 648-2402, CA aut NATIONALLICENCE 700 1- Pagé M. Laboratoire de cancérologie, Département de biochimie, Faculté de médecine, Université Laval, Québec G1K 7P4, Canada, CA aut NATIONALLICENCE 700 1- Yelle S. Centre de recherche en horticulture, Département de phytologie, FSAA, Université Laval, Québec G1K 7P4, Canada, CA aut NATIONALLICENCE 700 1- Laberge S. Agriculture et Agroalimentaire Canada, 2560 boul. Hochelage, Ste-Foy Québec G1V 2J3, Canada e-mail: laberges@em.agr.ca Tel.: +1-(418) 657-7980, Fax: +1-(418) 648-2402, CA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer