caa a22 4500 47579642X CHVBK 20180406123725.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s001220051354 doi (NATIONALLICENCE)springer-10.1007/s001220051354 Genetic analysis of temperature-sensitive male sterilty in rice [Elektronische Daten] [O. U. K. Reddy, E. A. Siddiq, N. P. Sarma, J. Ali, A. J. Hussain, P. Nimmakayala, P. Ramasamy, S. Pammi, A. S. Reddy] Abstract : The present study of genetic analysis is an attempt to precisely characterize diverse temperature-sensitive genic male-sterile (TGMS) lines so as to explore the possibilities of utilizing the most promising in large-scale hybrid seed production. Genetical studies revealed that the TGMS segregants derived from crosses involving TGMS lines ID24 and SA2 expressed differential fertility levels at low-temperature conditions. A majority of these progenies expressed transgressive segregation towards either sterility of fertility, causing instability of sterility and low reversibilty of fertility which may be due to large numbers of single-locus QTLs and their epistatic interactions. We identified two putative genes imparting temperature-sensitive male sterility after observing crosses involving diverse TGMS sources. To identify suitable molecular markers closely linked to the trait we used RAPD, AFLP and microsatellites which generated polymorphism through bulked segregant analysis. AFLP analysis using a smaller genome kit resulted in enormous polymorphism, out of which the combination EAA/MCAG amplified a 330-bp fragment, which closely segregated with the gene at a distance of 5.3 cM. This fragment was eluted for cloning and from the sequence a STS primer (TS200) was developed which produced a dominant polymorphism specific to TGMS. The microsatellite RM257, located earlier on chromosome 9, was linked with the TGMS trait in SA2 at a distance of 6.2 cM. RM257 produced a codominant polymorphism with 145-bp (sterile) and 132-bp (fertile) products. Both individually and collectively, the markers TS200 and RM257 located on either side of the TGMS locus are very useful for marker-assisted selection. Springer-Verlag Berlin Heidelberg, 2000 Key words TGMS nationallicence RAPD nationallicence AFLP nationallicence Microsatellites nationallicence STS nationallicence Marker-assisted selection nationallicence Bulked seg analysis nationallicence Reddy O. U. K. Crop Biotechnology Center, Texas A & M University, College Station, TX-77843-2123, USA, US aut Siddiq E. A. Directorate of Rice Research, Rajendra Nagar, Hyderabad, India-500 030, IN aut Sarma N. P. Directorate of Rice Research, Rajendra Nagar, Hyderabad, India-500 030, IN aut Ali J. Tamilnadu Agriculture University, Coimbatore, India-641 003, IN aut Hussain A. J. Directorate of Rice Research, Rajendra Nagar, Hyderabad, India-500 030, IN aut Nimmakayala P. Crop Biotechnology Center, Texas A & M University, College Station, TX-77843-2123, USA, US aut Ramasamy P. Crop Biotechnology Center, Texas A & M University, College Station, TX-77843-2123, USA, US aut Pammi S. Crop Biotechnology Center, Texas A & M University, College Station, TX-77843-2123, USA, US aut Reddy A. S. Dow Agro Sciences, 9330 Zionsville Road, Indianapolis, IN-46268-1054, USA, US aut https://doi.org/10.1007/s001220051354 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051354 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reddy O. U. K. Crop Biotechnology Center, Texas A & M University, College Station, TX-77843-2123, USA, US aut NATIONALLICENCE 700 1- Siddiq E. A. Directorate of Rice Research, Rajendra Nagar, Hyderabad, India-500 030, IN aut NATIONALLICENCE 700 1- Sarma N. P. Directorate of Rice Research, Rajendra Nagar, Hyderabad, India-500 030, IN aut NATIONALLICENCE 700 1- Ali J. Tamilnadu Agriculture University, Coimbatore, India-641 003, IN aut NATIONALLICENCE 700 1- Hussain A. J. Directorate of Rice Research, Rajendra Nagar, Hyderabad, India-500 030, IN aut NATIONALLICENCE 700 1- Nimmakayala P. Crop Biotechnology Center, Texas A & M University, College Station, TX-77843-2123, USA, US aut NATIONALLICENCE 700 1- Ramasamy P. Crop Biotechnology Center, Texas A & M University, College Station, TX-77843-2123, USA, US aut NATIONALLICENCE 700 1- Pammi S. Crop Biotechnology Center, Texas A & M University, College Station, TX-77843-2123, USA, US aut NATIONALLICENCE 700 1- Reddy A. S. Dow Agro Sciences, 9330 Zionsville Road, Indianapolis, IN-46268-1054, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer