caa a22 4500 475796438 CHVBK 20180406123725.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s001220051337 doi (NATIONALLICENCE)springer-10.1007/s001220051337 Detection of 5S and 25S rRNA genes in Sinapis alba, Raphanus sativus and Brassica napus by double fluorescence in situ hybridization [Elektronische Daten] [O. Schrader, H. Budahn, R. Ahne] Abstract : Different ribosomal RNA (5S and 25S) genes were investigated simultaneously by fluorescence in situ hybridization (FISH) in Sinapis alba, Raphanus sativus and Brassica napus. The chromosomes of S. alba carried four 5S and six 25S gene sites, and those of R. sativus four sites of each gene, respectively. These two species have one chromosome pair with both rDNA genes; the two are closely located on a short arm of S. alba, while in R. sativus one is distal on the short arm (5S) and the other more proximal on the long arm (25S). In B. napus we have confirmed 12sites of 25S rDNA. The detection of 5S rDNA genes revealed 14 signals on 12 chromosomes. Of these, six chromosomes had signals for both rDNA genes. The FISH with 5S rDNA probes detected two sites closely adjacent in four chromosomes of B napus. These results are discussed in relation to a probable homoeologous chromosome pair in B. oleracea. Springer-Verlag Berlin Heidelberg, 2000 Key words Brassica napus nationallicence Sinapis alba nationallicence Raphanus sativus nationallicence FISH nationallicence 5S rDNA genes nationallicence 25S rDNA genes nationallicence Schrader O. Federal Centre for Breeding Research on cultivated Plants, Institute for Breeding Methods in Vegetables, Neuer Weg 22/23, D-06484 Quedlinburg, Germany e-mail: o.schrader@bafz.de, Tel. +49-03936/47294 Fax: +49-03946/47255, DE aut Budahn H. Federal Centre for Breeding Research on cultivated Plants, Institute for Breeding Methods in Vegetables, Neuer Weg 22/23, D-06484 Quedlinburg, Germany e-mail: o.schrader@bafz.de, Tel. +49-03936/47294 Fax: +49-03946/47255, DE aut Ahne R. Federal Centre for Breeding Research on cultivated Plants, Institute for Breeding Methods in Vegetables, Neuer Weg 22/23, D-06484 Quedlinburg, Germany e-mail: o.schrader@bafz.de, Tel. +49-03936/47294 Fax: +49-03946/47255, DE aut https://doi.org/10.1007/s001220051337 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051337 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schrader O. Federal Centre for Breeding Research on cultivated Plants, Institute for Breeding Methods in Vegetables, Neuer Weg 22/23, D-06484 Quedlinburg, Germany e-mail: o.schrader@bafz.de, Tel. +49-03936/47294 Fax: +49-03946/47255, DE aut NATIONALLICENCE 700 1- Budahn H. Federal Centre for Breeding Research on cultivated Plants, Institute for Breeding Methods in Vegetables, Neuer Weg 22/23, D-06484 Quedlinburg, Germany e-mail: o.schrader@bafz.de, Tel. +49-03936/47294 Fax: +49-03946/47255, DE aut NATIONALLICENCE 700 1- Ahne R. Federal Centre for Breeding Research on cultivated Plants, Institute for Breeding Methods in Vegetables, Neuer Weg 22/23, D-06484 Quedlinburg, Germany e-mail: o.schrader@bafz.de, Tel. +49-03936/47294 Fax: +49-03946/47255, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer