caa a22 4500 475796446 CHVBK 20180406123725.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s001220051355 doi (NATIONALLICENCE)springer-10.1007/s001220051355 Cytosine deaminase as a substrate-dependent negative selectable marker in Brassica napus [Elektronische Daten] [A. V. Babwah, C. S. Waddell] Abstract : The enzyme cytosine deaminase, encoded by the codA gene, catalyzes the deamination of the non- toxic compound 5-fluorocytosine (5-FC) to the highly toxic compound 5-fluorouracil (5-FU). Cytosine deaminase activity is not found in higher plants and Brassica napus seedlings are unaffected by the presence of 5-FC in the growth medium. In codA-transformed B. napus seedlings, expression of cytosine deaminase results in a reduction of root and hypocotyl lengths, and a severe suppression of true leaf development. This phenotype is dependent on the presence of the 5-FC substrate and no effects are seen in plants grown in the absence of the substrate or in sibling plants lacking the transgene. The codA transformants have been assessed over three generations of growth and in each generation the transgene is stably inherited and confers the same 5-FC-sensitive phenotype. Transfer of 5-FC-sensitive seedlings to soil results in the restoration of normal growth in up to 100% of the seedlings. These results indicate that codA is a versatile dominant marker gene that can be used effectively in B. napus for substrate-dependent negative selection. Springer-Verlag Berlin Heidelberg, 2000 Key words Brassica napus nationallicence Cytosine deaminase nationallicence codA gene nationallicence 5-Fluorocytosine nationallicence Negative selection nationallicence Babwah A. V. McGill University, Department of Biology, 1205 Dr. Penfield Avenue, Montréal, Québec, H3A 1B1, Canada, CA aut Waddell C. S. McGill University, Department of Biology, 1205 Dr. Penfield Avenue, Montréal, Québec, H3A 1B1, Canada, CA aut https://doi.org/10.1007/s001220051355 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051355 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Babwah A. V. McGill University, Department of Biology, 1205 Dr. Penfield Avenue, Montréal, Québec, H3A 1B1, Canada, CA aut NATIONALLICENCE 700 1- Waddell C. S. McGill University, Department of Biology, 1205 Dr. Penfield Avenue, Montréal, Québec, H3A 1B1, Canada, CA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer