caa a22 4500 475796462 CHVBK 20180406123725.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s001220051342 doi (NATIONALLICENCE)springer-10.1007/s001220051342 Mapping and genome organization of microsatellite sequences in rice ( Oryza sativa L.) [Elektronische Daten] [S. Temnykh, William D. Park, Nicola Ayres, Sam Cartinhour, N. Hauck, L. Lipovich, Y. G. Cho, T. Ishii, S. R. McCouch] Abstract : In order to enhance the resolution of an existing genetic map of rice, and to obtain a comprehensive picture of marker utility and genomic distribution of microsatellites in this important grain species, rice DNA sequences containing simple sequence repeats (SSRs) were extracted from several small-insert genomic libraries and from the database. One hundred and eighty eight new microsatellite markers were developed and evaluated for allelic diversity. The new simple sequence length polymorphisms (SSLPs) were incorporated into the existing map previously containing 124 SSR loci. The 312 microsatellite markers reported here provide whole-genome coverage with an average density of one SSLP per 6 cM. In this study, 26 SSLP markers were identified in published sequences of known genes, 65 were developed based on partial cDNA sequences available in GenBank, and 97 were isolated from genomic libraries. Microsatellite markers with different SSR motifs are relatively uniformly distributed along rice chromosomes regardless of whether they were derived from genomic clones or cDNA sequences. However, the distribution of polymorphism detected by these markers varies between different regions of the genome. Springer-Verlag Berlin Heidelberg, 2000 Key words Microsatellite markers nationallicence Genetic map nationallicence Allelic diversity nationallicence Genome organization nationallicence Rice (Oryza sativa L.) nationallicence Temnykh S. Department of Plant Breeding, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USA e-mail: stm4@cornell.edu Tel.: +1 607 255 0420, US aut Park William D. Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77845, USA, US aut Ayres Nicola Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77845, USA, US aut Cartinhour Sam Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77845, USA, US aut Hauck N. Department of Plant Breeding, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USA e-mail: stm4@cornell.edu Tel.: +1 607 255 0420, US aut Lipovich L. Department of Plant Breeding, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USA e-mail: stm4@cornell.edu Tel.: +1 607 255 0420, US aut Cho Y. G. Department of Plant Breeding, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USA e-mail: stm4@cornell.edu Tel.: +1 607 255 0420, US aut Ishii T. Department of Plant Breeding, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USA e-mail: stm4@cornell.edu Tel.: +1 607 255 0420, US aut McCouch S. R. Department of Plant Breeding, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USA e-mail: stm4@cornell.edu Tel.: +1 607 255 0420, US aut https://doi.org/10.1007/s001220051342 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051342 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Temnykh S. Department of Plant Breeding, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USA e-mail: stm4@cornell.edu Tel.: +1 607 255 0420, US aut NATIONALLICENCE 700 1- Park William D. Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77845, USA, US aut NATIONALLICENCE 700 1- Ayres Nicola Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77845, USA, US aut NATIONALLICENCE 700 1- Cartinhour Sam Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77845, USA, US aut NATIONALLICENCE 700 1- Hauck N. Department of Plant Breeding, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USA e-mail: stm4@cornell.edu Tel.: +1 607 255 0420, US aut NATIONALLICENCE 700 1- Lipovich L. Department of Plant Breeding, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USA e-mail: stm4@cornell.edu Tel.: +1 607 255 0420, US aut NATIONALLICENCE 700 1- Cho Y. G. Department of Plant Breeding, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USA e-mail: stm4@cornell.edu Tel.: +1 607 255 0420, US aut NATIONALLICENCE 700 1- Ishii T. Department of Plant Breeding, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USA e-mail: stm4@cornell.edu Tel.: +1 607 255 0420, US aut NATIONALLICENCE 700 1- McCouch S. R. Department of Plant Breeding, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USA e-mail: stm4@cornell.edu Tel.: +1 607 255 0420, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer