caa a22 4500 475796470 CHVBK 20180406123726.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s001220051349 doi (NATIONALLICENCE)springer-10.1007/s001220051349 Expression of active barley seed ribosome-inactivating protein in transgenic wheat [Elektronische Daten] [S. Bieri, I. Potrykus, J. Fütterer] Abstract : Phenotypically normal, transgenic wheat (Triticum aestivum, var. Frisal) plants expressing a barley seed ribosome-inactivating protein (RIP) were produced. Expression was controlled by an intron-enhanced cauliflower mosaic virus 35S promoter and has been completely stable over four generations so far, possibly due to matrix-associated regions (MARs) that flank the transgenes. An engineered fusion to a signal peptide derived from the barley seed ß-1,3-glucanase caused the transport of RIP to the apoplast. Activity of the accumulated protein could be shown by significant inhibition of a rabbit reticulocyte transcription/translation system. Plants expressing high levels of RIP were protected only moderately or not at all against infection by the fungal pathogen Erysiphe graminis. Springer-Verlag Berlin Heidelberg, 2000 Key words Wheat transformation nationallicence Ribosome-inactivating protein nationallicence Anti-fungal protein nationallicence Protein-targeting nationallicence Bieri S. Institute of Plant Sciences, ETH Zürich, Universitätsstrasse 2, CH 8092 Zürich, Switzerland e-mail: johannes.fuetterer@ipw.biol.ethz.ch Fax: +41 1-632-10-44, CH aut Potrykus I. Institute of Plant Sciences, ETH Zürich, Universitätsstrasse 2, CH 8092 Zürich, Switzerland e-mail: johannes.fuetterer@ipw.biol.ethz.ch Fax: +41 1-632-10-44, CH aut Fütterer J. Institute of Plant Sciences, ETH Zürich, Universitätsstrasse 2, CH 8092 Zürich, Switzerland e-mail: johannes.fuetterer@ipw.biol.ethz.ch Fax: +41 1-632-10-44, CH aut https://doi.org/10.1007/s001220051349 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051349 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bieri S. Institute of Plant Sciences, ETH Zürich, Universitätsstrasse 2, CH 8092 Zürich, Switzerland e-mail: johannes.fuetterer@ipw.biol.ethz.ch Fax: +41 1-632-10-44, CH aut NATIONALLICENCE 700 1- Potrykus I. Institute of Plant Sciences, ETH Zürich, Universitätsstrasse 2, CH 8092 Zürich, Switzerland e-mail: johannes.fuetterer@ipw.biol.ethz.ch Fax: +41 1-632-10-44, CH aut NATIONALLICENCE 700 1- Fütterer J. Institute of Plant Sciences, ETH Zürich, Universitätsstrasse 2, CH 8092 Zürich, Switzerland e-mail: johannes.fuetterer@ipw.biol.ethz.ch Fax: +41 1-632-10-44, CH aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer