caa a22 4500 475796527 CHVBK 20180406123726.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s001220051344 doi (NATIONALLICENCE)springer-10.1007/s001220051344 Analysis of SSRs derived from grape ESTs [Elektronische Daten] [K. D. Scott, P. Eggler, G. Seaton, M. Rossetto, E. M. Ablett, L. S. Lee, R. J. Henry] Abstract : One hundred and twenty four microsatellites were isolated from analysis of 5000 Vitis expressed sequence tags (ESTs). A diversity of dinucleotide and trinucleotide simple sequence repeat (SSR) motifs were present. Primers were designed for 16 of these SSRs and they were tested on seven accessions. Ten of the sixteen primer pairs resulted in PCR products of the expected size. All ten functional primers were polymorphic across the accessions studied. Polymorphisms were evident at the level of cultivars, Vitis species, and between related genera. SSRs that were from the 3′ untranslated region (3′UTR) were most polymorphic at the cultivar level, the 5′ untranslated region (5′ UTR) SSRs were most polymorphic between cultivars and species, and those SSRs within coding sequence were most polymorphic between species and genera. These results show that EST-derived SSRs in Vitis are useful as they are polymorphic and highly transferable. With EST SSRs being applicable to studies at several taxonomic levels, the large number of SSRs (approximately 1000) that will be available from an expanded EST database of 45 000 will have many potential applications in mapping and identity research. Springer-Verlag Berlin Heidelberg, 2000 Key words ESTs nationallicence Microsatellites nationallicence Vitis vinifera nationallicence Scott K. D. Centre for Plant Conservation Genetics, PO Box 157, Lismore NSW 2480, Southern Cross University, Australia e-mail: kscott@scu.edu.au Tel.: +61 2 66203141, Fax: +61 2 66222080, AU aut Eggler P. Centre for Plant Conservation Genetics, PO Box 157, Lismore NSW 2480, Southern Cross University, Australia e-mail: kscott@scu.edu.au Tel.: +61 2 66203141, Fax: +61 2 66222080, AU aut Seaton G. Australian Agriculture Research Institute, PO Box 157, Lismore NSW 2480, Southern Cross University, Australia, AU aut Rossetto M. Australian Agriculture Research Institute, PO Box 157, Lismore NSW 2480, Southern Cross University, Australia, AU aut Ablett E. M. Centre for Plant Conservation Genetics, PO Box 157, Lismore NSW 2480, Southern Cross University, Australia e-mail: kscott@scu.edu.au Tel.: +61 2 66203141, Fax: +61 2 66222080, AU aut Lee L. S. Australian Agriculture Research Institute, PO Box 157, Lismore NSW 2480, Southern Cross University, Australia, AU aut Henry R. J. Centre for Plant Conservation Genetics, PO Box 157, Lismore NSW 2480, Southern Cross University, Australia e-mail: kscott@scu.edu.au Tel.: +61 2 66203141, Fax: +61 2 66222080, AU aut https://doi.org/10.1007/s001220051344 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051344 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Scott K. D. Centre for Plant Conservation Genetics, PO Box 157, Lismore NSW 2480, Southern Cross University, Australia e-mail: kscott@scu.edu.au Tel.: +61 2 66203141, Fax: +61 2 66222080, AU aut NATIONALLICENCE 700 1- Eggler P. Centre for Plant Conservation Genetics, PO Box 157, Lismore NSW 2480, Southern Cross University, Australia e-mail: kscott@scu.edu.au Tel.: +61 2 66203141, Fax: +61 2 66222080, AU aut NATIONALLICENCE 700 1- Seaton G. Australian Agriculture Research Institute, PO Box 157, Lismore NSW 2480, Southern Cross University, Australia, AU aut NATIONALLICENCE 700 1- Rossetto M. Australian Agriculture Research Institute, PO Box 157, Lismore NSW 2480, Southern Cross University, Australia, AU aut NATIONALLICENCE 700 1- Ablett E. M. Centre for Plant Conservation Genetics, PO Box 157, Lismore NSW 2480, Southern Cross University, Australia e-mail: kscott@scu.edu.au Tel.: +61 2 66203141, Fax: +61 2 66222080, AU aut NATIONALLICENCE 700 1- Lee L. S. Australian Agriculture Research Institute, PO Box 157, Lismore NSW 2480, Southern Cross University, Australia, AU aut NATIONALLICENCE 700 1- Henry R. J. Centre for Plant Conservation Genetics, PO Box 157, Lismore NSW 2480, Southern Cross University, Australia e-mail: kscott@scu.edu.au Tel.: +61 2 66203141, Fax: +61 2 66222080, AU aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer