caa a22 4500 475796535 CHVBK 20180406123726.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s001220051357 doi (NATIONALLICENCE)springer-10.1007/s001220051357 A secondary effect of transformation in Rhizobium leguminosarum transgenic for Bacillus thuringiensis subspecies tenebrionis δ-endotoxin ( cryIIIA ) genes. Part 2 [Elektronische Daten] [G. Giddings, L. Mytton, L. Taylor, S. Thomas, D. Allen, L. Skøt] Summary : Rhizobium leguminosarum strains were produced for the biological control of Sitona larvae by introducing Bacillus thuringiensis subspecies tenebrionis delta-endotoxin genes (cryIIIA). Comparisons between a transgenic and parent strain show that transformation has induced changes not associated with the intended function of the transgene. Although growth rates in laboratory cultures are similar for both strains the ability to compete for nodule sites is greater in the transgenic than in the non-transformed parent strain, a character that has remained stable over 4 years. This increased ability, which was previously observed in axenic culture, is shown here to also occur in non-sterile soil, although the effect is less pronounced than in sterile conditions. Experiments in soil show a highly significant difference from the expected nodule occupancy ratio, assuming no difference between genotypes and with no significant variation between replicates. These results demonstrate that the ecological and agronomic characters of transgenics might be unexpectedly altered by transformation. Such characters might have a bearing on the safety and/ or success of transgenics released into the environment. Springer-Verlag Berlin Heidelberg, 2000 Key words Rhizobium nationallicence Transformation nationallicence Nodule occupancy nationallicence Soil nationallicence Giddings G. Cledwyn Building, Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, SY23 3DD, Wales, UK e-mail: gdg@aber.ac.uk Tel: +44-01970 621764, Fax: +44-01970 622307, GB aut Mytton L. Cledwyn Building, Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, SY23 3DD, Wales, UK e-mail: gdg@aber.ac.uk Tel: +44-01970 621764, Fax: +44-01970 622307, GB aut Taylor L. Cledwyn Building, Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, SY23 3DD, Wales, UK e-mail: gdg@aber.ac.uk Tel: +44-01970 621764, Fax: +44-01970 622307, GB aut Thomas S. Cledwyn Building, Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, SY23 3DD, Wales, UK e-mail: gdg@aber.ac.uk Tel: +44-01970 621764, Fax: +44-01970 622307, GB aut Allen D. Cledwyn Building, Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, SY23 3DD, Wales, UK e-mail: gdg@aber.ac.uk Tel: +44-01970 621764, Fax: +44-01970 622307, GB aut Skøt L. Cledwyn Building, Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, SY23 3DD, Wales, UK e-mail: gdg@aber.ac.uk Tel: +44-01970 621764, Fax: +44-01970 622307, GB aut https://doi.org/10.1007/s001220051357 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051357 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Giddings G. Cledwyn Building, Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, SY23 3DD, Wales, UK e-mail: gdg@aber.ac.uk Tel: +44-01970 621764, Fax: +44-01970 622307, GB aut NATIONALLICENCE 700 1- Mytton L. Cledwyn Building, Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, SY23 3DD, Wales, UK e-mail: gdg@aber.ac.uk Tel: +44-01970 621764, Fax: +44-01970 622307, GB aut NATIONALLICENCE 700 1- Taylor L. Cledwyn Building, Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, SY23 3DD, Wales, UK e-mail: gdg@aber.ac.uk Tel: +44-01970 621764, Fax: +44-01970 622307, GB aut NATIONALLICENCE 700 1- Thomas S. Cledwyn Building, Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, SY23 3DD, Wales, UK e-mail: gdg@aber.ac.uk Tel: +44-01970 621764, Fax: +44-01970 622307, GB aut NATIONALLICENCE 700 1- Allen D. Cledwyn Building, Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, SY23 3DD, Wales, UK e-mail: gdg@aber.ac.uk Tel: +44-01970 621764, Fax: +44-01970 622307, GB aut NATIONALLICENCE 700 1- Skøt L. Cledwyn Building, Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, SY23 3DD, Wales, UK e-mail: gdg@aber.ac.uk Tel: +44-01970 621764, Fax: +44-01970 622307, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer