caa a22 4500 47579656X CHVBK 20180406123726.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s001220051345 doi (NATIONALLICENCE)springer-10.1007/s001220051345 Molecular mapping of a rice gene conditioning thermosensitive genic male sterility using AFLP, RFLP and SSR techniques [Elektronische Daten] [N. V. Dong, P. K. Subudhi, P. N. Luong, V. D. Quang, T. D. Quy, H. G. Zheng, B. Wang, H. T. Nguyen] Abstract : The discovery and application of the thermosensitive genic male sterility (TGMS) system has great potential for revolutionizing hybrid seed production technology in rice. Use of the TGMS system in two-line breeding is simple, inexpensive, efficient, and eliminates the limitations associated with the cytoplasmic-genetic male sterility (CMS) system. An F2 population developed from a cross between a TGMS indica mutant, TGMS-VN1, and a fertile indica line, CH1, was used to identify molecular markers linked to the TGMS gene and to subsequently determine its chromosomal location on the linkage map of rice. Bulk segregant analysis was performed using the AFLP technique. From the surveyof 200 AFLP primer combinations, four AFLP markers (E2/M5-600, E3/M16-400, E5/M12-600, and E5/M12-200) linked to the TGMS gene were identified. All the markers were linked to the gene in the coupling phase. All except E2/M5-200 were found to be low-copy sequences. However, the marker E5/M12-600 showed polymorphism in RFLP analysis and was closely linked to the TGMS gene at a distance of 3.3 cM. This marker was subsequently mapped on chromosome 2 using doubled-haploid mapping populations derived from the crosses IR64×Azucena and CT9993×IR62666, available at IRRI, Philippines, and Texas Tech University, respectively. Linkage of microsatellite marker RM27 with the TGMS gene further confirmed its location on chromosome 2. The closest marker, E5/M12-600, was sequenced so that a PCR marker can be developed for the marker-assisted transfer of this gene to different genetic backgrounds. The new TGMS gene is tentatively designated as tms4(t). Springer-Verlag Berlin Heidelberg, 2000 Key words AFLP nationallicence Bulked segregant analysis nationallicence Gene tagging nationallicence Marker-assisted selection nationallicence Rice TGMS gene nationallicence Dong N. V. Molecular Biology Laboratory, Agricultural Genetics Institute, Tulieum, Hanoi, Vietnam, VN aut Subudhi P. K. Plant Molecular Genetics Laboratory, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409-2122, USA e-mail: bwhtn@ttacs.ttu.edu Fax:+1-806-742-2888, US aut Luong P. N. Molecular Biology Laboratory, Agricultural Genetics Institute, Tulieum, Hanoi, Vietnam, VN aut Quang V. D. Molecular Biology Laboratory, Agricultural Genetics Institute, Tulieum, Hanoi, Vietnam, VN aut Quy T. D. Molecular Biology Laboratory, Agricultural Genetics Institute, Tulieum, Hanoi, Vietnam, VN aut Zheng H. G. Plant Molecular Genetics Laboratory, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409-2122, USA e-mail: bwhtn@ttacs.ttu.edu Fax:+1-806-742-2888, US aut Wang B. Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China, CN aut Nguyen H. T. Plant Molecular Genetics Laboratory, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409-2122, USA e-mail: bwhtn@ttacs.ttu.edu Fax:+1-806-742-2888, US aut https://doi.org/10.1007/s001220051345 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051345 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dong N. V. Molecular Biology Laboratory, Agricultural Genetics Institute, Tulieum, Hanoi, Vietnam, VN aut NATIONALLICENCE 700 1- Subudhi P. K. Plant Molecular Genetics Laboratory, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409-2122, USA e-mail: bwhtn@ttacs.ttu.edu Fax:+1-806-742-2888, US aut NATIONALLICENCE 700 1- Luong P. N. Molecular Biology Laboratory, Agricultural Genetics Institute, Tulieum, Hanoi, Vietnam, VN aut NATIONALLICENCE 700 1- Quang V. D. Molecular Biology Laboratory, Agricultural Genetics Institute, Tulieum, Hanoi, Vietnam, VN aut NATIONALLICENCE 700 1- Quy T. D. Molecular Biology Laboratory, Agricultural Genetics Institute, Tulieum, Hanoi, Vietnam, VN aut NATIONALLICENCE 700 1- Zheng H. G. Plant Molecular Genetics Laboratory, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409-2122, USA e-mail: bwhtn@ttacs.ttu.edu Fax:+1-806-742-2888, US aut NATIONALLICENCE 700 1- Wang B. Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China, CN aut NATIONALLICENCE 700 1- Nguyen H. T. Plant Molecular Genetics Laboratory, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409-2122, USA e-mail: bwhtn@ttacs.ttu.edu Fax:+1-806-742-2888, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer