caa a22 4500 475796640 CHVBK 20180406123726.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050025 doi (NATIONALLICENCE)springer-10.1007/s001220050025 High-resolution linkage analysis and physical characterization of the EIX-responding locus in tomato [Elektronische Daten] [M. Ron, R. Kantety, G. B. Martin, N. Avidan, Y. Eshed, D. Zamir, A. Avni] Abstract : An ethylene-inducing xylanase (EIX) from Tricohoderma viride is a potent elicitor of ethylene biosynthesis, localized cell death and other defense responses in specific cultivars of tobacco (Nicotiana tabacum) and tomato (Lycopersicon esculentum). Wild species of tomato, such as Lycopersicon cheesmanii and Lycopersicon pennellii, do not respond to EIX treatment. The F1 progeny of a L. esculentum×L. cheesmanii and a L. esculentum×L. pennellii cross responded to EIX treatment with an increase in ethylene biosynthesis and the induction of localized cell death. The F2 progeny of the above mentioned crosses segregated 3:1 (responding:non-responding). We mapped the EIX-responding locus (Eix) to the short arm of chromosome 7 using a population of introgression lines (ILs), containing small RFLP-defined chromosome segments of L. pennellii introgressed into L. esculentum. RFLP analysis of 990 F2 plants that segregated for the introgressed segment mapped the Eix locus 0.1 cM and 0.9 cM from the flanking markers TG61 and TG131, respectively. Using the marker TG61 we isolated a yeast artificial chromosome (YAC) clone that carries 300-kb DNA segments derived from the Eix region. By mapping the ends of this YAC clone we show that it spans the Eix locus. Thus, positional cloning of the Eix locus appears feasible. Springer-Verlag Berlin Heidelberg, 2000 Key words Map-based cloning nationallicence RFLP nationallicence YAC nationallicence Ron M. Department of Plant Sciences, Tel-Aviv University, Tel-Aviv, 69978, Israel e-mail: Ipavni@ccsg.tau.ac.il Tel.: 972 3 640 9840, Fax: 972 3-640 9380, IL aut Kantety R. Boyce Thompson Institute, Tower Road, Ithaca, NY 14853, USA, US aut Martin G. B. Boyce Thompson Institute, Tower Road, Ithaca, NY 14853, USA, US aut Avidan N. Department of Plant Sciences, Tel-Aviv University, Tel-Aviv, 69978, Israel e-mail: Ipavni@ccsg.tau.ac.il Tel.: 972 3 640 9840, Fax: 972 3-640 9380, IL aut Eshed Y. Department of Field and Vegetable Crops, Hebrew University, Rehovot, 76100, Israel, IL aut Zamir D. Department of Field and Vegetable Crops, Hebrew University, Rehovot, 76100, Israel, IL aut Avni A. Department of Plant Sciences, Tel-Aviv University, Tel-Aviv, 69978, Israel e-mail: Ipavni@ccsg.tau.ac.il Tel.: 972 3 640 9840, Fax: 972 3-640 9380, IL aut https://doi.org/10.1007/s001220050025 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050025 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ron M. Department of Plant Sciences, Tel-Aviv University, Tel-Aviv, 69978, Israel e-mail: Ipavni@ccsg.tau.ac.il Tel.: 972 3 640 9840, Fax: 972 3-640 9380, IL aut NATIONALLICENCE 700 1- Kantety R. Boyce Thompson Institute, Tower Road, Ithaca, NY 14853, USA, US aut NATIONALLICENCE 700 1- Martin G. B. Boyce Thompson Institute, Tower Road, Ithaca, NY 14853, USA, US aut NATIONALLICENCE 700 1- Avidan N. Department of Plant Sciences, Tel-Aviv University, Tel-Aviv, 69978, Israel e-mail: Ipavni@ccsg.tau.ac.il Tel.: 972 3 640 9840, Fax: 972 3-640 9380, IL aut NATIONALLICENCE 700 1- Eshed Y. Department of Field and Vegetable Crops, Hebrew University, Rehovot, 76100, Israel, IL aut NATIONALLICENCE 700 1- Zamir D. Department of Field and Vegetable Crops, Hebrew University, Rehovot, 76100, Israel, IL aut NATIONALLICENCE 700 1- Avni A. Department of Plant Sciences, Tel-Aviv University, Tel-Aviv, 69978, Israel e-mail: Ipavni@ccsg.tau.ac.il Tel.: 972 3 640 9840, Fax: 972 3-640 9380, IL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer