caa a22 4500 475796659 CHVBK 20180406123726.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050030 doi (NATIONALLICENCE)springer-10.1007/s001220050030 Evolutionary features of chondriome divergence in Triticum (wheat) and Aegilops shown by RFLP analysis of mitochondrial DNAs [Elektronische Daten] [G. -Z. Wang, Y. Matsuoka, K. Tsunewaki] Abstract : The first comprehensive analysis was made of restriction fragment length polymorphism (RFLP) of the mitochondrial (mt) DNA of two related genera, Triticum (wheat) and Aegilops. This led to clarification of the nature of mtDNA variability and the inference of the phylogeny of the mitochondrial genomes (=chondriome). Forty-six alloplasmic lines and one euplasmic line of common wheat (2n = 42, genomes AABBDD) carrying plasmons (cytoplasmic genomes) of 47 accessions belonging to 33 species were used. This consisted of nearly all the Triticum and Aegilops species. RFLP analysis, carried out with seven mitochondrial gene probes (7.0 kb in total) in combination with three restriction endonucleases, found marked variation: Of the 168 bands detected, 165 were variable (98.2%), indicative that there is extremely high mtDNA variability in these genera. This high variability is attributed to the variation present in the intergenic regions. Most of the variation was between chondriomes of different plasmon types; only 8 bands (4.8%) between those of the same plasmon types were variable, evidence of clear chondriome divergence between different plasmon types. The first comprehensive phylogenetic trees of the chondriome were constructed on the basis of genetic distances. All but 1 of the polyploids had chondriomes closely related to those of 1 putative parent, indicative of uniparental chondriome transmission at the time of polyploid formation. The chondriome showed parallel evolutionary divergence to the plastome (chloroplast genome). Use of a minimum set of 3 mtDNA probe-enzyme combinations is proposed for tentative plasmon type identification and the screening of new plasmon types in those genera. Springer-Verlag Berlin Heidelberg, 2000 Key words Mitochondrial DNA nationallicence RFLP analysis nationallicence Triticum nationallicence Aegilops nationallicence Plasmon phylogeny nationallicence Wang G. -Z Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjyojima, Matsuoka, Yoshida-gun, Fukui 910-1195, Japan e-mail: tunewaki@fpu.ac.jp Fax: 81-776-61-6011, JP aut Matsuoka Y. Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjyojima, Matsuoka, Yoshida-gun, Fukui 910-1195, Japan e-mail: tunewaki@fpu.ac.jp Fax: 81-776-61-6011, JP aut Tsunewaki K. Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjyojima, Matsuoka, Yoshida-gun, Fukui 910-1195, Japan e-mail: tunewaki@fpu.ac.jp Fax: 81-776-61-6011, JP aut https://doi.org/10.1007/s001220050030 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050030 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang G. -Z Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjyojima, Matsuoka, Yoshida-gun, Fukui 910-1195, Japan e-mail: tunewaki@fpu.ac.jp Fax: 81-776-61-6011, JP aut NATIONALLICENCE 700 1- Matsuoka Y. Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjyojima, Matsuoka, Yoshida-gun, Fukui 910-1195, Japan e-mail: tunewaki@fpu.ac.jp Fax: 81-776-61-6011, JP aut NATIONALLICENCE 700 1- Tsunewaki K. Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjyojima, Matsuoka, Yoshida-gun, Fukui 910-1195, Japan e-mail: tunewaki@fpu.ac.jp Fax: 81-776-61-6011, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer