caa a22 4500 475796667 CHVBK 20180406123726.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050024 doi (NATIONALLICENCE)springer-10.1007/s001220050024 The interspecific genome structure of cultivated banana, Musa spp [Elektronische Daten] revealed by genomic DNA in situ hybridization [A. D'Hont, A. Paget-Goy, J. Escoute, F. Carreel] Abstract : In cultivated banana, Musa spp., there are four known genomes, A, B, S, and T. These correspond to the genetic constitutions of wild Eumusa species M. acuminata, M. balbisiana, M. schizocarpa and the Australimusa species, respectively. Most cultivated clones are triploid or diploid, they have been classified into genomic groups according to chromosome numbers and morphological traits. Genomic in situ hybridization (GISH) enabled us to differentiate the chromosomes of these four genomes; however, a distal portion of the chromosomes remained unlabelled. GISH was used to determine the exact genome structure of interspecific cultivated clones.In most cases the results were consistent with the chromosome constitution estimated by means of phenotypic descriptors.The one notable exception, the clone 'Pelipita', has the 8 A and 25 B chromosomes instead of the predicted 11 A and 22 B. GISH also enabled us to determine the chromosome complement of a few clones that could not be classified only on the basis of phenotypic descriptors and chromosome counts. The rDNA sites were located in Musa species. They appeared to be often associated with satellites, which can be separated from the chromosomes, representing a potential source of error for chromosome counting using classical techniques. Springer-Verlag Berlin Heidelberg, 2000 Key words Musa spp nationallicence Banana nationallicence Genomic in situ hybridization nationallicence Aneuploidy nationallicence Polyploidy nationallicence rDNA genes nationallicence D'Hont A. CIRAD, BP 5035, 34032 Montpellier Cedex 1, France Fax: 33 4 67615605 e-mail: dhont@cirad.fr, FR aut Paget-Goy A. CIRAD, BP 5035, 34032 Montpellier Cedex 1, France Fax: 33 4 67615605 e-mail: dhont@cirad.fr, FR aut Escoute J. CIRAD, BP 5035, 34032 Montpellier Cedex 1, France Fax: 33 4 67615605 e-mail: dhont@cirad.fr, FR aut Carreel F. Cirad Station de Neufchateau, 97130 Capesterre-Belle-Eau, France, FR aut https://doi.org/10.1007/s001220050024 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050024 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- D'Hont A. CIRAD, BP 5035, 34032 Montpellier Cedex 1, France Fax: 33 4 67615605 e-mail: dhont@cirad.fr, FR aut NATIONALLICENCE 700 1- Paget-Goy A. CIRAD, BP 5035, 34032 Montpellier Cedex 1, France Fax: 33 4 67615605 e-mail: dhont@cirad.fr, FR aut NATIONALLICENCE 700 1- Escoute J. CIRAD, BP 5035, 34032 Montpellier Cedex 1, France Fax: 33 4 67615605 e-mail: dhont@cirad.fr, FR aut NATIONALLICENCE 700 1- Carreel F. Cirad Station de Neufchateau, 97130 Capesterre-Belle-Eau, France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer