caa a22 4500 475796705 CHVBK 20180406123726.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050029 doi (NATIONALLICENCE)springer-10.1007/s001220050029 The nad4L-orf25 gene cluster is conserved and expressed in sugar beet mitochondria [Elektronische Daten] [T. Kubo, M. P. Yamamoto, T. Mikami] Abstract : We have found that a gene coding for NADH dehydrogenase subunit 4L and a presumed gene, orf25, are linked and co-transcribed with each other in sugar beet mitochondria. Ten and twelve C-to-U editing events were observed in the mRNAs of nad4L and orf25, respectively; the amino-acid sequence specified after editing is better-conserved in comparison with the homologues of other organisms. It is interesting to note that the translation initiation codon of nad4L is created by editing. The conservation of the nad4L-orf25 linkage was examined by PCR-amplification of the intergenic region. We obtained successful PCR products from five dicots (spinach, apple, snapdragon, petunia and tobacco) and two monocots (tulip and pineapple), but not in two poaceous plants, rice and maize. The intergenic region, when present, was found to be well-conserved in its sequence, suggesting a monophyletic origin of this linkage. Our result, together with previous reports of Arabidopsis and four poaceous species, favour the argument that the nad4L-orf25 linkage is conserved throughout angiosperms except in the Poaceae. Springer-Verlag Berlin Heidelberg, 2000 Key words Nad4L-orf25 linkage nationallicence RNA editing nationallicence Sugar beet nationallicence Mitochondria nationallicence Kubo T. Laboratory of Genetic Engineering, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan e-mail: gelab@abs.agr.hokudai.ac.jp Fax: +81 11 716 0879, JP aut Yamamoto M. P. Laboratory of Genetic Engineering, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan e-mail: gelab@abs.agr.hokudai.ac.jp Fax: +81 11 716 0879, JP aut Mikami T. Laboratory of Genetic Engineering, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan e-mail: gelab@abs.agr.hokudai.ac.jp Fax: +81 11 716 0879, JP aut https://doi.org/10.1007/s001220050029 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050029 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kubo T. Laboratory of Genetic Engineering, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan e-mail: gelab@abs.agr.hokudai.ac.jp Fax: +81 11 716 0879, JP aut NATIONALLICENCE 700 1- Yamamoto M. P. Laboratory of Genetic Engineering, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan e-mail: gelab@abs.agr.hokudai.ac.jp Fax: +81 11 716 0879, JP aut NATIONALLICENCE 700 1- Mikami T. Laboratory of Genetic Engineering, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan e-mail: gelab@abs.agr.hokudai.ac.jp Fax: +81 11 716 0879, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer