caa a22 4500 475796748 CHVBK 20180406123726.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050039 doi (NATIONALLICENCE)springer-10.1007/s001220050039 Application of AFLP, RAPD and ISSR markers to genetic mapping of European and Japanese larch [Elektronische Daten] [A. Arcade, F. Anselin, P. Faivre Rampant, M. C. Lesage, L. E. Pâques, D. Prat] Abstract : Genetic linkage maps have been increasingly developed for a wide variety of plants, using segregating populations such as F2s or backcrosses between inbred lines. These pedigrees are rarely available in outbred species like forest trees which have long generation times. Thus genetic mapping studies have to use peculiar pedigrees and markers in appropriate configurations. We constructed single-tree genetic linkage maps of European larch (Larix decidua Mill.) and Japanese larch [Larix kaempferi (Lamb.) Carr.] using segregation data from 112 progeny individuals of an hybrid family. A total of 266 markers (114 AFLP, 149 RAPD and 3 ISSR loci) showing a testcross configuration, i.e.heterozygous in one parent and null in the other parent, were grouped at LOD 4.0, θ=0.3. The maternal parent map (L. decidua)consisted of 117 markers partitioned within 17 linkage groups (1152 cM) and the paternal parent map (L. kaempferi) had 125 markers assembled into 21 linkage groups (1206 cM). The map distance covered by markers was determined by adding a 34.7-cM independence distance at the end of each group and unlinked marker. It reached 2537 cM and 2997 cM respectively for European larch and Japanese larch, and represented respectively a 79.6% and 80.8% coverage of the overall genome. A few 3:1 segregating markers were used to identify homologous linkage groups between the European larch and the Japanese larch genetic maps. The PCR-based molecular markers allowed the construction of genetic maps, thus ensuring a good coverage of the larch genome for further QTL detection and mapping studies. Springer-Verlag Berlin Heidelberg, 2000 Keywords Larix nationallicence Linkage map nationallicence RAPD nationallicence AFLP nationallicence ISSR nationallicence Genetic mapping nationallicence Arcade A. INRA, Unité Amélioration, Génétique et Physiologie forestières, Avenue de la Pomme de Pin, BP 20619 Ardon, F-45166 Olivet Cedex, France e-mail: prat@orleans.inra.fr Tel.: +33-2 38 41 78 16 Fax: +33-2 38 41 78 79, FR aut Anselin F. INRA, Unité Amélioration, Génétique et Physiologie forestières, Avenue de la Pomme de Pin, BP 20619 Ardon, F-45166 Olivet Cedex, France e-mail: prat@orleans.inra.fr Tel.: +33-2 38 41 78 16 Fax: +33-2 38 41 78 79, FR aut Rampant P. Faivre INRA, Unité Amélioration, Génétique et Physiologie forestières, Avenue de la Pomme de Pin, BP 20619 Ardon, F-45166 Olivet Cedex, France e-mail: prat@orleans.inra.fr Tel.: +33-2 38 41 78 16 Fax: +33-2 38 41 78 79, FR aut Lesage M. C. INRA, Unité Amélioration, Génétique et Physiologie forestières, Avenue de la Pomme de Pin, BP 20619 Ardon, F-45166 Olivet Cedex, France e-mail: prat@orleans.inra.fr Tel.: +33-2 38 41 78 16 Fax: +33-2 38 41 78 79, FR aut Pâques L. E. INRA, Unité Amélioration, Génétique et Physiologie forestières, Avenue de la Pomme de Pin, BP 20619 Ardon, F-45166 Olivet Cedex, France e-mail: prat@orleans.inra.fr Tel.: +33-2 38 41 78 16 Fax: +33-2 38 41 78 79, FR aut Prat D. INRA, Unité Amélioration, Génétique et Physiologie forestières, Avenue de la Pomme de Pin, BP 20619 Ardon, F-45166 Olivet Cedex, France e-mail: prat@orleans.inra.fr Tel.: +33-2 38 41 78 16 Fax: +33-2 38 41 78 79, FR aut https://doi.org/10.1007/s001220050039 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050039 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arcade A. INRA, Unité Amélioration, Génétique et Physiologie forestières, Avenue de la Pomme de Pin, BP 20619 Ardon, F-45166 Olivet Cedex, France e-mail: prat@orleans.inra.fr Tel.: +33-2 38 41 78 16 Fax: +33-2 38 41 78 79, FR aut NATIONALLICENCE 700 1- Anselin F. INRA, Unité Amélioration, Génétique et Physiologie forestières, Avenue de la Pomme de Pin, BP 20619 Ardon, F-45166 Olivet Cedex, France e-mail: prat@orleans.inra.fr Tel.: +33-2 38 41 78 16 Fax: +33-2 38 41 78 79, FR aut NATIONALLICENCE 700 1- Rampant P. Faivre INRA, Unité Amélioration, Génétique et Physiologie forestières, Avenue de la Pomme de Pin, BP 20619 Ardon, F-45166 Olivet Cedex, France e-mail: prat@orleans.inra.fr Tel.: +33-2 38 41 78 16 Fax: +33-2 38 41 78 79, FR aut NATIONALLICENCE 700 1- Lesage M. C. INRA, Unité Amélioration, Génétique et Physiologie forestières, Avenue de la Pomme de Pin, BP 20619 Ardon, F-45166 Olivet Cedex, France e-mail: prat@orleans.inra.fr Tel.: +33-2 38 41 78 16 Fax: +33-2 38 41 78 79, FR aut NATIONALLICENCE 700 1- Pâques L. E. INRA, Unité Amélioration, Génétique et Physiologie forestières, Avenue de la Pomme de Pin, BP 20619 Ardon, F-45166 Olivet Cedex, France e-mail: prat@orleans.inra.fr Tel.: +33-2 38 41 78 16 Fax: +33-2 38 41 78 79, FR aut NATIONALLICENCE 700 1- Prat D. INRA, Unité Amélioration, Génétique et Physiologie forestières, Avenue de la Pomme de Pin, BP 20619 Ardon, F-45166 Olivet Cedex, France e-mail: prat@orleans.inra.fr Tel.: +33-2 38 41 78 16 Fax: +33-2 38 41 78 79, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer