caa a22 4500 475796764 CHVBK 20180406123726.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050035 doi (NATIONALLICENCE)springer-10.1007/s001220050035 Organisation and expression of mitochondrial atp9 genes from CMS and fertile carrots [Elektronische Daten] [M. Szklarczyk, M. Oczkowski, H. Augustyniak, T. Börner, B. Linke, B. Michalik] Abstract : The F0-F1 ATPase subunit 9 gene of carrot mitochondria has been isolated from both petaloid male-sterile (atp9-1) and normal fertile cytoplasms (atp9-3). The position occupied in atp9-3 by the TAA stop codon is, in the case of atp9-1 replaced by the CAA triplet coding for glutamine, which makes this latter ORF 13 amino acid residues longer than that of the atp9-3. The 3' end of atp9-3 gene is flanked by a direct repeat of 42 bp. The sequence of the repeat unit is also present at the 3' end of atp9-1 but without reiteration. A truncated and presumably inactive version of atp9 (atp9-2) was found to be present in cytoplasms regardless of the fertility phenotype which they condition. The atp9-1 gene from petaloid cytoplasm appeared to be co-transcribed with the gene coding for 5S rRNA, and nuclear background influenced the accumulation of the respective transcript. The results are discussed with respect to a potential role of atp9-1 in generating the petaloid form of CMS. Springer-Verlag Berlin Heidelberg, 2000 Key words Cytoplasmic male sterility nationallicence F0-F1 ATPase subunit 9 nationallicence Mitochondrial DNA nationallicence Carrot nationallicence Daucus carota nationallicence Szklarczyk M. Department of Genetics, Plant Breeding and Seed Science, Cracow Agricultural University, Al. 29-go Listopada 54, 31-425 Cracow, Poland Fax: +48 12-411-13-22, e-mail: ubszklar@cyf-kr.edu.pl, PL aut Oczkowski M. Department of Plant Molecular Biology, Miedzychodzka 5, 60-371 Poznan, Poland, PL aut Augustyniak H. Department of Plant Molecular Biology, Miedzychodzka 5, 60-371 Poznan, Poland, PL aut Börner T. Institute of Biology (Genetics), Humboldt University, Chausseestr. 117, D-10115 Berlin, Germany, DE aut Linke B. Institute of Biology (Genetics), Humboldt University, Chausseestr. 117, D-10115 Berlin, Germany, DE aut Michalik B. aut https://doi.org/10.1007/s001220050035 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050035 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Szklarczyk M. Department of Genetics, Plant Breeding and Seed Science, Cracow Agricultural University, Al. 29-go Listopada 54, 31-425 Cracow, Poland Fax: +48 12-411-13-22, e-mail: ubszklar@cyf-kr.edu.pl, PL aut NATIONALLICENCE 700 1- Oczkowski M. Department of Plant Molecular Biology, Miedzychodzka 5, 60-371 Poznan, Poland, PL aut NATIONALLICENCE 700 1- Augustyniak H. Department of Plant Molecular Biology, Miedzychodzka 5, 60-371 Poznan, Poland, PL aut NATIONALLICENCE 700 1- Börner T. Institute of Biology (Genetics), Humboldt University, Chausseestr. 117, D-10115 Berlin, Germany, DE aut NATIONALLICENCE 700 1- Linke B. Institute of Biology (Genetics), Humboldt University, Chausseestr. 117, D-10115 Berlin, Germany, DE aut NATIONALLICENCE 700 1- Michalik B. aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer