caa a22 4500 475796780 CHVBK 20180406123726.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050034 doi (NATIONALLICENCE)springer-10.1007/s001220050034 Fgr , a major locus that modulates the fructose to glucose ratio in mature tomato fruits [Elektronische Daten] [I. Levin, N. Gilboa, E. Yeselson, S. Shen, A. A. Schaffer] Abstract : A genetic trait determining the ratio of fructose to glucose in mature tomato fruits is described. A backcross breeding program based on the interspecific cross of Lycopersicon hirsutum and L. esculentum yielded stable genotypes with a high ratio of fructose to glucose (>1.5:1) compared with the approximately equimolar ratios found in L. esculentum. Two inter-simple- sequence repeat (ISSR) DNA sequences, highly associated (20 <LOD score <21) with the trait, were identified. The markers were found to be less associated with either glucose or fructose levels individually (2 <LOD score <3) and were statistically unlinked to total sugars and total soluble solids (TSS). These two ISSR bands segregated in a dominant fashion and were found to be allelic to each other, one associated in coupling and the other in repulsion with the trait of high fructose to glucose ratio. Both ISSR markers were mapped to the centromeric region of tomato chromosome 4. Quantitative analysis of the identified locus, based on data from segregating F2, BC and F3 populations from the cross between genotypes having high and low fructose to glucose ratios, suggested that the L. hirsutum-derived allele (Fgr H), which increases the fructose to glucose ratio, is partially dominant. Fgr H leads to an increase in fructose levels and a subsequent decrease in glucose levels, with no effect on total hexose levels. Accordingly, we conclude that the Fgr locus modulates the partitioning of hexose sugars between fructose and glucose, with no effect on total sugars or TSS. Springer-Verlag Berlin Heidelberg, 2000 Key words Lycopersicon hirsutum nationallicence Lycopersicon esculentum nationallicence PCR nationallicence Fructose nationallicence Glucose nationallicence Levin I. Department of Plant Genetics and Breeding, Institute of Field and Garden Crops, The Volcani Center, P.O. Box 6, Bet Dagan, Israel 50250 e-mail: VCLEVINI@AGRI.GOV.IL Fax: +972 3 9669642, IL aut Gilboa N. Department of Plant Genetics and Breeding, Institute of Field and Garden Crops, The Volcani Center, P.O. Box 6, Bet Dagan, Israel 50250 e-mail: VCLEVINI@AGRI.GOV.IL Fax: +972 3 9669642, IL aut Yeselson E. Department of Vegetable Crops, Institute of Field and Garden Crops, The Volcani Center, P.O. Box 6, Bet Dagan, Israel 50250, IL aut Shen S. Department of Vegetable Crops, Institute of Field and Garden Crops, The Volcani Center, P.O. Box 6, Bet Dagan, Israel 50250, IL aut Schaffer A. A. Department of Vegetable Crops, Institute of Field and Garden Crops, The Volcani Center, P.O. Box 6, Bet Dagan, Israel 50250, IL aut https://doi.org/10.1007/s001220050034 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050034 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Levin I. Department of Plant Genetics and Breeding, Institute of Field and Garden Crops, The Volcani Center, P.O. Box 6, Bet Dagan, Israel 50250 e-mail: VCLEVINI@AGRI.GOV.IL Fax: +972 3 9669642, IL aut NATIONALLICENCE 700 1- Gilboa N. Department of Plant Genetics and Breeding, Institute of Field and Garden Crops, The Volcani Center, P.O. Box 6, Bet Dagan, Israel 50250 e-mail: VCLEVINI@AGRI.GOV.IL Fax: +972 3 9669642, IL aut NATIONALLICENCE 700 1- Yeselson E. Department of Vegetable Crops, Institute of Field and Garden Crops, The Volcani Center, P.O. Box 6, Bet Dagan, Israel 50250, IL aut NATIONALLICENCE 700 1- Shen S. Department of Vegetable Crops, Institute of Field and Garden Crops, The Volcani Center, P.O. Box 6, Bet Dagan, Israel 50250, IL aut NATIONALLICENCE 700 1- Schaffer A. A. Department of Vegetable Crops, Institute of Field and Garden Crops, The Volcani Center, P.O. Box 6, Bet Dagan, Israel 50250, IL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer