caa a22 4500 475796799 CHVBK 20180406123726.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050023 doi (NATIONALLICENCE)springer-10.1007/s001220050023 The distribution, organization and evolution of two abundant and widespread repetitive DNA sequences in the genus Hordeum [Elektronische Daten] [S. Taketa, H. Ando, K. Takeda, G. E. Harrison, J. S. Heslop-Harrison] Abstract : The genomic organization and chromosomal distributions of two abundant tandemly repeated DNA sequences, dpTa1 and pSc119.2, were examined in six wild Hordeum taxa, representing the four basic genomes of the genus, by Southern and fluorescence in situ hybridization. The dpTa1 probe hybridized to between 30 and 60 sites on the chromosomes of all five diploid species studied, but hybridization patterns differed among the species. Hybridization of the pSc119.2 sequence to the chromosomes and Southern blots of digested DNA detected signals in Hordeum bulbosum, Hordeum chilense, Hordeum marinum and Hordeum murinum 4x, but not in Hordeum murinum 2x and Hordeum vulgare ssp. spontaneum. A maximum of one pSc119.2 signal was observed in the terminal or subterminal region of each chromosome arm in the species carrying this sequence. The species carrying the same I-genome differed in the presence (Hordeum bulbosum) or absence (Hordeum spontaneum) of pSc119.2. The presence of pSc119.2 in the tetraploid cytotype of Hordeum murinum, but its absence in the diploid cytotype, suggests that the tetraploid is not likely to be a simple autotetraploid of the diploid. Data about the inter- and intra-specific variation of the two independent repetitive DNA sequences give information about both the interrelationships of the species and the evolution of the repetitive sequences. Springer-Verlag Berlin Heidelberg, 2000 Key words Barley nationallicence Hordeum nationallicence In situ hybridization nationallicence Phylogeny nationallicence Tandemly repeated DNA sequence nationallicence Taketa S. Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan, JP aut Ando H. Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan, JP aut Takeda K. Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan, JP aut Harrison G. E. Department of Cell Biology, John Innes Centre, UK, GB aut Heslop-Harrison J. S. Department of Cell Biology, John Innes Centre, UK, GB aut https://doi.org/10.1007/s001220050023 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050023 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Taketa S. Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan, JP aut NATIONALLICENCE 700 1- Ando H. Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan, JP aut NATIONALLICENCE 700 1- Takeda K. Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan, JP aut NATIONALLICENCE 700 1- Harrison G. E. Department of Cell Biology, John Innes Centre, UK, GB aut NATIONALLICENCE 700 1- Heslop-Harrison J. S. Department of Cell Biology, John Innes Centre, UK, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer