caa a22 4500 475796829 CHVBK 20180406123727.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050032 doi (NATIONALLICENCE)springer-10.1007/s001220050032 Application of microsatellites in wheat ( Triticum aestivum L.) for studying genetic differentiation caused by selection for adaptation and use [Elektronische Daten] [M. Stachel, T. Lelley, H. Grausgruber, J. Vollmann] Abstract : For studying genetic differentiation caused by selection for adaptation and end-use, the allele frequencies of 42 microsatellites (MS), representative of the three wheat genomes, were analysed in a total of 60 wheat cultivars. The cultivars originate from three agroecological areas (AEAs) - Germany, Austria and Hungary - and represent equal numbers of 'quality wheats' and 'feed wheats' for each country. For the 42 loci, 202 alleles were detected using PAGE and silver staining. The average number of alleles per locus was 4.8, including four monomorphic loci. For 16 loci, null alleles were detected. Cluster analysis clearly differentiated the varieties according to the three AEAs and, within each AEA, into quality wheats from feed wheats. Analysis of variance revealed highly significant differences of distance data between AEAs as well as between quality groups. The correlation between genetic distance (GD) and pedigree data (coefficient of diversity, COD) was r s=0.45. The results have proven the excellent resolving power of MS in varietal differentiation, which arises through breeding under specific environmental conditions, and for different end-use. Springer-Verlag Berlin Heidelberg, 2000 Key words DNA markers nationallicence Genetic diversity nationallicence Agroecological area nationallicence Quality nationallicence Adaptation nationallicence Stachel M. Institute for Agrobiotechnology, Department of Plant Biotechnology, Konrad Lorenz Strasse 20, A-3430 Tulln, Austria e-mail: lelley@ifa-tulln.ac.at, AT aut Lelley T. Institute for Agrobiotechnology, Department of Plant Biotechnology, Konrad Lorenz Strasse 20, A-3430 Tulln, Austria e-mail: lelley@ifa-tulln.ac.at, AT aut Grausgruber H. University of Agricultural Sciences Vienna, Department of Plant Breeding, Gregor Mendel Strasse 33, A-1180 Vienna, Austria, AT aut Vollmann J. University of Agricultural Sciences Vienna, Department of Plant Breeding, Gregor Mendel Strasse 33, A-1180 Vienna, Austria, AT aut https://doi.org/10.1007/s001220050032 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050032 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stachel M. Institute for Agrobiotechnology, Department of Plant Biotechnology, Konrad Lorenz Strasse 20, A-3430 Tulln, Austria e-mail: lelley@ifa-tulln.ac.at, AT aut NATIONALLICENCE 700 1- Lelley T. Institute for Agrobiotechnology, Department of Plant Biotechnology, Konrad Lorenz Strasse 20, A-3430 Tulln, Austria e-mail: lelley@ifa-tulln.ac.at, AT aut NATIONALLICENCE 700 1- Grausgruber H. University of Agricultural Sciences Vienna, Department of Plant Breeding, Gregor Mendel Strasse 33, A-1180 Vienna, Austria, AT aut NATIONALLICENCE 700 1- Vollmann J. University of Agricultural Sciences Vienna, Department of Plant Breeding, Gregor Mendel Strasse 33, A-1180 Vienna, Austria, AT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer