caa a22 4500 475796837 CHVBK 20180406123727.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050042 doi (NATIONALLICENCE)springer-10.1007/s001220050042 Silencing of HMW glutenins in transgenic wheat expressing extra HMW subunits [Elektronische Daten] [M. L. Alvarez, S. Guelman, N. G. Halford, S. Lustig, M. I. Reggiardo, N. Ryabushkina, P. Schewry, J. Stein, R. H. Vallejos] Abstract : Wheat HMW glutenin subunit genes 1Ax1 and 1Dx5 were introduced, and either expressed or overexpressed, into a commercial wheat cultivar that already expresses five subunits. Six independent transgenic events were obtained and characterized by SDS-PAGE and Southern analysis. The 1Dx5 gene was overexpressed in two events without changes in the other endosperm proteins. Overexpression of 1Dx5 increased the contribution of HMW glutenin subunits to total protein up to 22%. Two events express the 1Ax1 subunit transgene with associated silencing of the 1Ax2* endogenous subunit. In the SDS-PAGE one of them shows a new HMW glutenin band of an apparent Mr lower than that of the 1Dx5 subunit. Southern analysis of the four events confirmed transformation and suggest that the transgenes are present in a low copy number. Silencing of all the HMW glutenin subunits was observed in two different events of transgenic wheat expressing the 1Ax1 subunit transgene and overexpressing the Dx5 gene. Transgenes and expression patterns were stably transmitted to the progenies in all the events except one where in some of the segregating T2 seeds the silencing of all HMW glutenin subunits was reverted associated with a drastic lost of transgenes from a high to a low copy number. The revertant T2 seeds expressed the five endogenous subunits plus the 1Ax1 transgene. Springer-Verlag Berlin Heidelberg, 2000 Key words Transgenic wheat nationallicence HMW glutenins nationallicence Gene silencing nationallicence Bread-making quality nationallicence Alvarez M. L. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), CONICET-UNR, Suipacha 531, 2000 Rosario, Argentina e-mail: cefobi@arnet.com.ar Tel.: 54 341 437-1955, Fax: 54 341 437-0044, AR aut Guelman S. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), CONICET-UNR, Suipacha 531, 2000 Rosario, Argentina e-mail: cefobi@arnet.com.ar Tel.: 54 341 437-1955, Fax: 54 341 437-0044, AR aut Halford N. G. Department of Agricultural Sciences, University of Bristol, AFRC Institute of Arable Crops Research, Long Ashton Research Station, Long Ashton, Bristol BS18 9AF, UK, GB aut Lustig S. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), CONICET-UNR, Suipacha 531, 2000 Rosario, Argentina e-mail: cefobi@arnet.com.ar Tel.: 54 341 437-1955, Fax: 54 341 437-0044, AR aut Reggiardo M. I. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), CONICET-UNR, Suipacha 531, 2000 Rosario, Argentina e-mail: cefobi@arnet.com.ar Tel.: 54 341 437-1955, Fax: 54 341 437-0044, AR aut Ryabushkina N. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), CONICET-UNR, Suipacha 531, 2000 Rosario, Argentina e-mail: cefobi@arnet.com.ar Tel.: 54 341 437-1955, Fax: 54 341 437-0044, AR aut Schewry P. Department of Agricultural Sciences, University of Bristol, AFRC Institute of Arable Crops Research, Long Ashton Research Station, Long Ashton, Bristol BS18 9AF, UK, GB aut Stein J. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), CONICET-UNR, Suipacha 531, 2000 Rosario, Argentina e-mail: cefobi@arnet.com.ar Tel.: 54 341 437-1955, Fax: 54 341 437-0044, AR aut Vallejos R. H. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), CONICET-UNR, Suipacha 531, 2000 Rosario, Argentina e-mail: cefobi@arnet.com.ar Tel.: 54 341 437-1955, Fax: 54 341 437-0044, AR aut https://doi.org/10.1007/s001220050042 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050042 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alvarez M. L. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), CONICET-UNR, Suipacha 531, 2000 Rosario, Argentina e-mail: cefobi@arnet.com.ar Tel.: 54 341 437-1955, Fax: 54 341 437-0044, AR aut NATIONALLICENCE 700 1- Guelman S. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), CONICET-UNR, Suipacha 531, 2000 Rosario, Argentina e-mail: cefobi@arnet.com.ar Tel.: 54 341 437-1955, Fax: 54 341 437-0044, AR aut NATIONALLICENCE 700 1- Halford N. G. Department of Agricultural Sciences, University of Bristol, AFRC Institute of Arable Crops Research, Long Ashton Research Station, Long Ashton, Bristol BS18 9AF, UK, GB aut NATIONALLICENCE 700 1- Lustig S. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), CONICET-UNR, Suipacha 531, 2000 Rosario, Argentina e-mail: cefobi@arnet.com.ar Tel.: 54 341 437-1955, Fax: 54 341 437-0044, AR aut NATIONALLICENCE 700 1- Reggiardo M. I. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), CONICET-UNR, Suipacha 531, 2000 Rosario, Argentina e-mail: cefobi@arnet.com.ar Tel.: 54 341 437-1955, Fax: 54 341 437-0044, AR aut NATIONALLICENCE 700 1- Ryabushkina N. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), CONICET-UNR, Suipacha 531, 2000 Rosario, Argentina e-mail: cefobi@arnet.com.ar Tel.: 54 341 437-1955, Fax: 54 341 437-0044, AR aut NATIONALLICENCE 700 1- Schewry P. Department of Agricultural Sciences, University of Bristol, AFRC Institute of Arable Crops Research, Long Ashton Research Station, Long Ashton, Bristol BS18 9AF, UK, GB aut NATIONALLICENCE 700 1- Stein J. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), CONICET-UNR, Suipacha 531, 2000 Rosario, Argentina e-mail: cefobi@arnet.com.ar Tel.: 54 341 437-1955, Fax: 54 341 437-0044, AR aut NATIONALLICENCE 700 1- Vallejos R. H. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), CONICET-UNR, Suipacha 531, 2000 Rosario, Argentina e-mail: cefobi@arnet.com.ar Tel.: 54 341 437-1955, Fax: 54 341 437-0044, AR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer