caa a22 4500 475799488 CHVBK 20180406123733.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1023/A:1002090627601 doi (NATIONALLICENCE)springer-10.1023/A:1002090627601 Behavioral Characterization and Genetic Analysis of the Drosophila melanogaster Larval Response to Light as Revealed by a Novel Individual Assay [Elektronische Daten] [Jana Hassan, Macarena Busto, Balaji Iyengar, Ana Campos] A new assay was designed, named checker, that measures the individual response to light in the fruitfly Drosophila melanogaster larva. In this assay the Drosophila larva apparently modulates its pattern of locomotion when faced with a choice between a dark and lit environment by orienting its movement towards the dark environment. We show that, in this assay, a response to light can be measured as an increase in residence time in the dark versus the lit quadrant. Mutations that disrupt phototransduction in the adult Drosophila abolish the larval response to light, demonstrating that this larval visual function is similar to that of the adult fly. Similarly, no response to light was detected in strains where the larval visual system (photoreceptors and target area) was disrupted by a mutation in the homeobox containing gene sine oculis (so) gene. Ablation of photoreceptors by the targeted expression of the cell death gene hid under the control of the photoreceptor-specific transcription factor glass (gl) abolishes this response entirely. Finally, we demonstrate that this response to light can be mediated by rhodopsins other than the blue absorbing Rh1. Plenum Publishing Corporation, 2000 Insect nationallicence larval photobehavior nationallicence locomotion nationallicence Drosophila nationallicence behavioral mutants nationallicence Hassan Jana Department of Biology, McMaster University, 1280 Main Street West, L8S 4K1, Hamilton, Ontario, Canada aut Busto Macarena Department of Biology, McMaster University, 1280 Main Street West, L8S 4K1, Hamilton, Ontario, Canada aut Iyengar Balaji Department of Biology, McMaster University, 1280 Main Street West, L8S 4K1, Hamilton, Ontario, Canada aut Campos Ana Department of Biology, McMaster University, 1280 Main Street West, L8S 4K1, Hamilton, Ontario, Canada aut Behavior Genetics Kluwer Academic Publishers-Plenum Publishers 30/1(2000-01-01), 59-69 0001-8244 30:1<59 2000 30 10519 https://doi.org/10.1023/A:1002090627601 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002090627601 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hassan Jana Department of Biology, McMaster University, 1280 Main Street West, L8S 4K1, Hamilton, Ontario, Canada aut NATIONALLICENCE 700 1- Busto Macarena Department of Biology, McMaster University, 1280 Main Street West, L8S 4K1, Hamilton, Ontario, Canada aut NATIONALLICENCE 700 1- Iyengar Balaji Department of Biology, McMaster University, 1280 Main Street West, L8S 4K1, Hamilton, Ontario, Canada aut NATIONALLICENCE 700 1- Campos Ana Department of Biology, McMaster University, 1280 Main Street West, L8S 4K1, Hamilton, Ontario, Canada aut NATIONALLICENCE 773 0- Behavior Genetics Kluwer Academic Publishers-Plenum Publishers 30/1(2000-01-01), 59-69 0001-8244 30:1<59 2000 30 10519 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer