caa a22 4500 47579964X CHVBK 20180406123733.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1023/A:1001910107167 doi (NATIONALLICENCE)springer-10.1023/A:1001910107167 Confirmation of Contextual Fear Conditioning QTLs by Short-Term Selection [Elektronische Daten] [Richard Radcliffe, Mark Lowe, Jeanne Wehner] A short-term selection study for contextual fear conditioning was conducted as a confirmational strategy to analyze the chromosomal locations of five previously mapped contextual fear conditioning quantitative trait loci (QTLs). The founding population was a C57BL/6 (B6) × DBA/2 (D2) F2 intercross. High and low lines were selected for three generations based on contextual fear conditioning scores. Fear conditioning was quantified as the percentage of time spent in a "frozen” posture when placed back into the chamber, where a mild footshock and a tone had been paired with exposure to the context 24 h earlier. Allele frequencies of at least three SSLP DNA markers linked to each of the five QTLs were determined in each generation. As the selection progressed, the frequency of D2 alleles decreased in the low line and increased in the high line for chromosomes 1 and 16, while the opposite was observed in chromosomes 2, 3, and 10. The direction of divergence for alleles on these five chromosomes is consistent with the original QTL mapping study. Differences between the lines in D2 allele frequencies were found to be significant for markers on chromosomes 2, 3, and 16 but did not reach significance on chromosomes 1 or 10. In general, the results are in good agreement with our original fear conditioning QTL mapping study and provide further evidence that these QTLs regulate variation in contextual fear conditioning in crosses of B6 and D2 mice. Plenum Publishing Corporation, 2000 QTL nationallicence selection nationallicence learning nationallicence fear conditioning nationallicence linkage nationallicence Radcliffe Richard Institute for Behavioral Genetics, University of Colorado, 80303, Boulder, Colorado aut Lowe Mark Institute for Behavioral Genetics, University of Colorado, 80303, Boulder, Colorado aut Wehner Jeanne Institute for Behavioral Genetics, University of Colorado, 80303, Boulder, Colorado aut Behavior Genetics Kluwer Academic Publishers-Plenum Publishers 30/3(2000-05-01), 183-191 0001-8244 30:3<183 2000 30 10519 https://doi.org/10.1023/A:1001910107167 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1001910107167 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Radcliffe Richard Institute for Behavioral Genetics, University of Colorado, 80303, Boulder, Colorado aut NATIONALLICENCE 700 1- Lowe Mark Institute for Behavioral Genetics, University of Colorado, 80303, Boulder, Colorado aut NATIONALLICENCE 700 1- Wehner Jeanne Institute for Behavioral Genetics, University of Colorado, 80303, Boulder, Colorado aut NATIONALLICENCE 773 0- Behavior Genetics Kluwer Academic Publishers-Plenum Publishers 30/3(2000-05-01), 183-191 0001-8244 30:3<183 2000 30 10519 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer