caa a22 4500 475799658 CHVBK 20180406123734.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1023/A:1001963906258 doi (NATIONALLICENCE)springer-10.1023/A:1001963906258 Identification and Time Dependence of Quantitative Trait Loci for Basal Locomotor Activity in the BXD Recombinant Inbred Series and a B6D2 F2 Intercross [Elektronische Daten] [Jay Koyner, Kristin Demarest, James McCaughran Jr, Laura Cipp, Robert Hitzemann] A complimentary two-phase strategy was used to detect and map quantitative trait loci (QTLs) associated with the basal locomotor response to a saline challenge (10 ml/kg). In phase 1, putative QTLs, significant at p < 0.01 or better, were identified by analysis of the strain means for 25 strains of the B × D recombinant inbred series. QTLs were identified on chromosomes 1, 3, 5, 9, 10, 16, and 18. Some of these QTLs were detected across the entire experimental period (0-20 min), while others were associated with specific 5-min blocks. Eighteen hundred C57BL/6J (B6) × DBA/2J (D2) F2 intercross animals were phenotyped for the basal locomotor response, and of this group, 500 to 700 individuals, psuedo-randomly selected, were used for a genomewide scan to confirm the RI-generated QTLs and to detect new QTLs. No new QTLs were detected but the QTLs on chromosome 1 were confirmed at p < 10−5 to p < 10−9, depending on the time interval. In addition, the QTLs on chromosomes 5 and 9 were confirmed at p < 0.001, providing a combined probability (RI + F2) which exceeds the threshold for a significant association. Two additional phenotypes which showed significant RI strain differences were examined—adaptation and thigmotaxis. Adaptation mapped to the same region of chromosome 9 and thigmotaxis to the same region of chromosome 1 as the distance-traveled QTL. Overall, the data presented here and elsewhere (Flint et al., 1995; Gershenfeld et al., 1997) illustrate that QTLs for basal activity are both robust and reliable. Plenum Publishing Corporation, 2000 Mouse nationallicence genes nationallicence recombinant inbred nationallicence QTL nationallicence activity nationallicence Koyner Jay Department of Psychiatry, SUNY of Stony Brook, 11794-8101, Stony Brook, New York aut Demarest Kristin Department of Psychiatry, SUNY of Stony Brook, 11794-8101, Stony Brook, New York aut McCaughran Jr James Department of Psychiatry, SUNY of Stony Brook, 11794-8101, Stony Brook, New York aut Cipp Laura Department of Psychiatry, SUNY of Stony Brook, 11794-8101, Stony Brook, New York aut Hitzemann Robert Department of Psychiatry, SUNY of Stony Brook, 11794-8101, Stony Brook, New York aut Behavior Genetics Kluwer Academic Publishers-Plenum Publishers 30/3(2000-05-01), 159-170 0001-8244 30:3<159 2000 30 10519 https://doi.org/10.1023/A:1001963906258 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1001963906258 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koyner Jay Department of Psychiatry, SUNY of Stony Brook, 11794-8101, Stony Brook, New York aut NATIONALLICENCE 700 1- Demarest Kristin Department of Psychiatry, SUNY of Stony Brook, 11794-8101, Stony Brook, New York aut NATIONALLICENCE 700 1- McCaughran Jr James Department of Psychiatry, SUNY of Stony Brook, 11794-8101, Stony Brook, New York aut NATIONALLICENCE 700 1- Cipp Laura Department of Psychiatry, SUNY of Stony Brook, 11794-8101, Stony Brook, New York aut NATIONALLICENCE 700 1- Hitzemann Robert Department of Psychiatry, SUNY of Stony Brook, 11794-8101, Stony Brook, New York aut NATIONALLICENCE 773 0- Behavior Genetics Kluwer Academic Publishers-Plenum Publishers 30/3(2000-05-01), 159-170 0001-8244 30:3<159 2000 30 10519 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer