caa a22 4500 475799925 CHVBK 20180406123734.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1023/A:1002796404278 doi (NATIONALLICENCE)springer-10.1023/A:1002796404278 Loss of Aggression, After Transfer onto a C57BL/6J Background, in Mice Carrying a Targeted Disruption of the Neuronal Nitric Oxide Synthase Gene [Elektronische Daten] [Isabelle Le Roy, Stéphanie Pothion, Stephane Mortaud, Caroline Chabert, Laurent Nicolas, Améziane Cherfouh, Pierre Roubertoux] Phenotypic differences among mice with disrupted genes and those with wild-type alleles have not provided the necessary evidence for desired gene/phenotype correlations. These differences could be due to "passenger genes" from the donor 129 strains that are used to produce stem cells. Three variations of attack behavior were measured, using mice carrying a disruption of the neural nitric oxide synthase gene. In the first population, the disrupted gene had been maintained on a mixed background including C57BL/6J and 129 alleles. We have developed a second population in which the disrupted gene was transferred onto a C57BL/6J background during five backcross generations. On the mixed C57BL/6J-129 background, mice homozygous for disrupted Nos1 alleles attacked more frequently, had shorter attack latencies, and presented a greater number of attacks than mice carrying nondisrupted alleles. On the C57BL/6J background, no significant difference persisted between the carriers of the disrupted gene and their noncarrier siblings. The noncarriers on the mixed C57BL/6J-129 background, and the carriers or noncarriers on the C57BL/6J background, did not differ from C57BL/6J. The frequency of attacking males was identical in the homozygous carriers of the disrupted gene, in the mixed C57BL/6J-129 background, and in the 129/SvPas, which approximates the 129/SvJae strain from which the stem cells were derived to produce the disrupted Nos1 gene. These results suggest that Nos1 disruption was not implicated in attack behavior. A possible passenger-gene effect from the 129 donor strain is discussed. Plenum Publishing Corporation, 2000 Gene-targeting nationallicence passenger genes nationallicence neural nitric oxide synthase nationallicence Nos1 nationallicence C57BL/6J nationallicence 129/SvPas nationallicence aggression nationallicence mice nationallicence Le Roy Isabelle aut Pothion Stéphanie aut Mortaud Stephane aut Chabert Caroline aut Nicolas Laurent aut Cherfouh Améziane aut Roubertoux Pierre aut Behavior Genetics Kluwer Academic Publishers-Plenum Publishers 30/5(2000-09-01), 367-373 0001-8244 30:5<367 2000 30 10519 https://doi.org/10.1023/A:1002796404278 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002796404278 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Le Roy Isabelle aut NATIONALLICENCE 700 1- Pothion Stéphanie aut NATIONALLICENCE 700 1- Mortaud Stephane aut NATIONALLICENCE 700 1- Chabert Caroline aut NATIONALLICENCE 700 1- Nicolas Laurent aut NATIONALLICENCE 700 1- Cherfouh Améziane aut NATIONALLICENCE 700 1- Roubertoux Pierre aut NATIONALLICENCE 773 0- Behavior Genetics Kluwer Academic Publishers-Plenum Publishers 30/5(2000-09-01), 367-373 0001-8244 30:5<367 2000 30 10519 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer