caa a22 4500 475799984 CHVBK 20180406123734.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1023/A:1001955205117 doi (NATIONALLICENCE)springer-10.1023/A:1001955205117 Identification of Quantitative Trait Loci Influencing Alcohol Consumption in the High Alcohol Drinking and Low Alcohol Drinking Rat Lines [Elektronische Daten] [Tatiana Foroud, Paula Bice, Peter Castelluccio, Ronghai Bo, Laurie Miller, Aimee Ritchotte, Lawrence Lumeng, Ting-Kai Li, Lucinda Carr] Selective breeding has been employed to develop high-alcohol-drinking (HAD) and low-alcohol-drinking (LAD) rat lines from the heterogeneous N/Nih rat. Within-family selection and a rotational breeding design were used to discourage inbreeding (Li et al, 1993). To identify quantitative trait loci (QTLs) contributing to alcohol consumption, reciprocal HAD and LAD matings in conjunction with F1 intercrosses were used to create 459 F2 progeny. Using selective genotyping of 151 F2 progeny with extreme alcohol consumption scores and a novel least squares method developed by Haley et al (1994), five chromosomal regions (1,5,10,12, and 16) were identified with lod scores greater than 2.0. Genotyping of the entire sample of 459 F2 progeny produced maximum lod scores of 3.5 on chromosome 5, 2.4 on chromosome 10, 4.7 on chromosome 12 and 2.9 on chromosome 16. The evidence of linkage to chromosome 1 diminished substantially to a maximum lod score of 0.5 when all F2 progeny were genotyped. This study is the first genome-wide study for QTLs underlying alcohol consumption that has employed noninbred lines. Further localization of these QTLs will likely provide insight and candidate genes for the study of human alcoholism. Plenum Publishing Corporation, 2000 Quantitative trait locus (QTL) nationallicence alcoholism nationallicence noninbred rat lines nationallicence Foroud Tatiana Departments of Medical and Molecular Genetics, USA aut Bice Paula Medicine, USA aut Castelluccio Peter Departments of Medical and Molecular Genetics, USA aut Bo Ronghai Medicine, USA aut Miller Laurie Medicine, USA aut Ritchotte Aimee Medicine, USA aut Lumeng Lawrence Medicine. Biochemistry and Molecular Biology, USA aut Li Ting-Kai Medicine. Biochemistry and Molecular Biology, USA aut Carr Lucinda Medicine. Pharmacology, Indiana University School of Medicine, 46202, Indianapolis, IN aut Behavior Genetics Kluwer Academic Publishers-Plenum Publishers 30/2(2000-03-01), 131-140 0001-8244 30:2<131 2000 30 10519 https://doi.org/10.1023/A:1001955205117 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1001955205117 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Foroud Tatiana Departments of Medical and Molecular Genetics, USA aut NATIONALLICENCE 700 1- Bice Paula Medicine, USA aut NATIONALLICENCE 700 1- Castelluccio Peter Departments of Medical and Molecular Genetics, USA aut NATIONALLICENCE 700 1- Bo Ronghai Medicine, USA aut NATIONALLICENCE 700 1- Miller Laurie Medicine, USA aut NATIONALLICENCE 700 1- Ritchotte Aimee Medicine, USA aut NATIONALLICENCE 700 1- Lumeng Lawrence Medicine. Biochemistry and Molecular Biology, USA aut NATIONALLICENCE 700 1- Li Ting-Kai Medicine. Biochemistry and Molecular Biology, USA aut NATIONALLICENCE 700 1- Carr Lucinda Medicine. Pharmacology, Indiana University School of Medicine, 46202, Indianapolis, IN aut NATIONALLICENCE 773 0- Behavior Genetics Kluwer Academic Publishers-Plenum Publishers 30/2(2000-03-01), 131-140 0001-8244 30:2<131 2000 30 10519 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer