caa a22 4500 475801199 CHVBK 20180406123737.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s002689910014 doi (NATIONALLICENCE)springer-10.1007/s002689910014 DNA Ploidy Status and Proliferative Activity as Markers of Malignant Potential in Barrett's Esophagus: Flow Cytometric Study Using Routinely Paraffin-embedded Tissue [Elektronische Daten] [Alberto Giménez, Alfredo Minguela, Luisa M. de Haro, Pascual Parrilla, Juan Bermejo, Domingo Pérez, Ana M. García, María A. Ortiz, Joaquín Molina, Rocío Álvarez] Regular endoscopic surveillance is recommended for patients with Barrett's esophagus to detect dysplasia and to diagnose carcinoma while it is in an early and possibly treatable stage. However, there are numerous unknown aspects regarding the natural history of dysplasia in this disease, and there is still a need for more accurate markers of risk of a malignant change. The aim of this study was to investigate the usefulness of DNA flow cytometry in Barrett's esophagus to define subgroups of patients showing similar histologic findings but with a different malignancy potential. Routinely formalin-fixed and paraffin-embedded tissues of 43 patients with Barrett's esophagus were processed for flow cytometric measurements (ploidy, proliferative index) and the results were compared with the histologic evolution observed in these patients. Only in the group of patients with "indefinite” dysplasia did we find statistically significant differences between the samples from patients with and without progression to more severe lesions (mean proliferative index of stable patients: 5.2% versus 8.3% in patients with progression, p= 0.001, Student's t-test). The presence in the flow cytometric analysis of a DNA aneuploid cell line is closely related to the presence of severe histologic alterations (i.e., high-grade dysplasia: p < 0.001, Fisher's exact test). Our results suggest that this procedure is at least capable of distinguishing between a real, although incipient, neoplastic process and morphologic changes of a reactive or reparative type. The increment in the tissue proliferative index could be an indicator of an early genomic instability which, with time, will develop into lesions with a more altered DNA content: aneuploidy. by the Société Internationale de Chirurgie, 2000 Giménez Alberto Department of Pathology, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut Minguela Alfredo Department of Immunology, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut de Haro Luisa M. Department of Surgery, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut Parrilla Pascual Department of Surgery, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut Bermejo Juan Department of Pathology, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut Pérez Domingo Department of Biostatistics, Faculty of Medicine, University of Murcia, Campus de Espinardo, s/n, 30080 Murcia, Spain, ES aut García Ana M. Department of Immunology, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut Ortiz María A. Department of Surgery, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut Molina Joaquín Department of Gastroenterology, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut Álvarez Rocío Department of Immunology, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut World Journal of Surgery Springer-Verlag 24/1(2000-01-01), 72-77 0364-2313 24:1<72 2000 24 268 https://doi.org/10.1007/s002689910014 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689910014 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Giménez Alberto Department of Pathology, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut NATIONALLICENCE 700 1- Minguela Alfredo Department of Immunology, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut NATIONALLICENCE 700 1- de Haro Luisa M. Department of Surgery, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut NATIONALLICENCE 700 1- Parrilla Pascual Department of Surgery, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut NATIONALLICENCE 700 1- Bermejo Juan Department of Pathology, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut NATIONALLICENCE 700 1- Pérez Domingo Department of Biostatistics, Faculty of Medicine, University of Murcia, Campus de Espinardo, s/n, 30080 Murcia, Spain, ES aut NATIONALLICENCE 700 1- García Ana M. Department of Immunology, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut NATIONALLICENCE 700 1- Ortiz María A. Department of Surgery, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut NATIONALLICENCE 700 1- Molina Joaquín Department of Gastroenterology, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut NATIONALLICENCE 700 1- Álvarez Rocío Department of Immunology, Virgen de la Arrixaca University Hospital, 30120 El Palmar, Murcia, Spain, ES aut NATIONALLICENCE 773 0- World Journal of Surgery Springer-Verlag 24/1(2000-01-01), 72-77 0364-2313 24:1<72 2000 24 268 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer