caa a22 4500 475801318 CHVBK 20180406123738.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s002689910010 doi (NATIONALLICENCE)springer-10.1007/s002689910010 Lidocaine-metabolizing Activity after Warm Ischemia and Reperfusion of the Rat Liver In Vivo [Elektronische Daten] [Kunihiko Izuishi, Hisao Wakabayashi, Takashi Maeba, Munemasa Ryu, Hajime Maeta] The effect of warm ischemia on lidocaine-metabolizing activity was examined in vivo. Total liver ischemia was produced for 1 hr in Sprague-Dawley rats by clamping the portal vein and hepatic artery at the hilum. Livers were then reperfused, and liver microsomes were prepared before and 0, 2, 6, and 24 hr, and 3, 6, and 10 days after reperfusion. Microsomal lidocaine-metabolizing activity and cytochrome P-450 content were examined. Lidocaine N-deethylase activity was decreased from 2.25 ± 0.33 to 0.97 ± 0.21 nmol/mg protein/min (mean ± SD) 24 hr after reperfusion. This inhibition was prolonged, and activity gradually recovered after 10 days. The cytochrome P-450 content showed the same tendency. On the other hand, serum levels of alanine aminotransferase increased significantly 2 hr after reperfusion and returned to control levels 3 days after reperfusion. Liver blood flow recovered rapidly after unclamping and reached baseline levels within 6 hr. Our results suggest that after warm ischemia, prolonged hepatic dysfunction in drug metabolism, which cannot be detected by evaluating serum enzymes or liver blood flow, exists at the microsomal level. by the Société Internationale de Chirurgie, 2000 Izuishi Kunihiko Department of Surgery, National Cancer Center Hospital East, 6-5-1, Kashiwanoha, Kashiwa, Chiba 277-8577, Japan, JP aut Wakabayashi Hisao First Department of Surgery, Kagawa Medical University, 1750-1, Miki-cho, Kita-gun, Kagawa 761-0701, Japan, JP aut Maeba Takashi First Department of Surgery, Kagawa Medical University, 1750-1, Miki-cho, Kita-gun, Kagawa 761-0701, Japan, JP aut Ryu Munemasa Department of Surgery, National Cancer Center Hospital East, 6-5-1, Kashiwanoha, Kashiwa, Chiba 277-8577, Japan, JP aut Maeta Hajime First Department of Surgery, Kagawa Medical University, 1750-1, Miki-cho, Kita-gun, Kagawa 761-0701, Japan, JP aut World Journal of Surgery Springer-Verlag 24/1(2000-01-01), 49-53 0364-2313 24:1<49 2000 24 268 https://doi.org/10.1007/s002689910010 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689910010 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Izuishi Kunihiko Department of Surgery, National Cancer Center Hospital East, 6-5-1, Kashiwanoha, Kashiwa, Chiba 277-8577, Japan, JP aut NATIONALLICENCE 700 1- Wakabayashi Hisao First Department of Surgery, Kagawa Medical University, 1750-1, Miki-cho, Kita-gun, Kagawa 761-0701, Japan, JP aut NATIONALLICENCE 700 1- Maeba Takashi First Department of Surgery, Kagawa Medical University, 1750-1, Miki-cho, Kita-gun, Kagawa 761-0701, Japan, JP aut NATIONALLICENCE 700 1- Ryu Munemasa Department of Surgery, National Cancer Center Hospital East, 6-5-1, Kashiwanoha, Kashiwa, Chiba 277-8577, Japan, JP aut NATIONALLICENCE 700 1- Maeta Hajime First Department of Surgery, Kagawa Medical University, 1750-1, Miki-cho, Kita-gun, Kagawa 761-0701, Japan, JP aut NATIONALLICENCE 773 0- World Journal of Surgery Springer-Verlag 24/1(2000-01-01), 49-53 0364-2313 24:1<49 2000 24 268 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer