caa a22 4500 475801350 CHVBK 20180406123738.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s002689910003 doi (NATIONALLICENCE)springer-10.1007/s002689910003 Activation of Monocytes and Endothelial Cells Depends on the Severity of Surgical Stress [Elektronische Daten] [Suefumi Aosasa, Satoshi Ono, Hidetaka Mochizuki, Hironori Tsujimoto, Shun-ichi Osada, Eiji Takayama, Shuhji Seki, Hoshio Hiraide] Surgical injury not only induces a systemic endocrine-metabolic response but also influences the function of the leukocytes and endothelial cells leading to various systemic responses. These responses appear to depend on the severity of surgical stress, which differs according to the surgical procedures. In this study, we investigated the response of monocytes and endothelial cells, and the development of systemic inflammatory response syndrome (SIRS) in relation to the severity of surgical stress. The postoperative clinical course was evaluated between patients undergoing an esophagectomy (ER group) and a distal gastrectomy (DG group). The tumor necrosis factor α (TNFα) production of monocytes, the serum interleukin 6 (IL-6) levels, the CD11b expression on either monocytes or granulocytes, and the intercellular adhesion molecule-1 (ICAM-1) expression on human umbilical vein endothelial cells (HUVECs) stimulated with culture supernatants of monocytes were compared between the 2 groups. The development of SIRS was observed in all patients in the ER group, whereas no patients demonstrated SIRS in the DG group. The serum IL-6 levels, TNFα production of monocytes, and CD11b intensity on monocytes or granulocytes in the ER group were higher than those in the DG group. In the ER group, the ICAM-1 intensity on HUVECs with monocytes immediately after operation significantly increased compared with before the operation. In conclusion, both the CD11b expression on monocytes and the TNFα production of monocytes are considered to reflect the degree of surgical stress, and the activation of endothelial cells stimulated with these activated leukocytes may therefore lead to both tissue and organ injury. by the Société Internationale de Chirurgie, 2000 Aosasa Suefumi Department of Surgery I, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut Ono Satoshi Department of Surgery I, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut Mochizuki Hidetaka Department of Surgery I, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut Tsujimoto Hironori Department of Surgery I, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut Osada Shun-ichi Department of Surgery I, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut Takayama Eiji Department of Parasitology, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut Seki Shuhji Division of Basic Traumatology, National Defense Medical College Research Institute, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut Hiraide Hoshio Division of Basic Traumatology, National Defense Medical College Research Institute, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut World Journal of Surgery Springer-Verlag 24/1(2000-01-01), 10-16 0364-2313 24:1<10 2000 24 268 https://doi.org/10.1007/s002689910003 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002689910003 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aosasa Suefumi Department of Surgery I, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut NATIONALLICENCE 700 1- Ono Satoshi Department of Surgery I, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut NATIONALLICENCE 700 1- Mochizuki Hidetaka Department of Surgery I, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut NATIONALLICENCE 700 1- Tsujimoto Hironori Department of Surgery I, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut NATIONALLICENCE 700 1- Osada Shun-ichi Department of Surgery I, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut NATIONALLICENCE 700 1- Takayama Eiji Department of Parasitology, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut NATIONALLICENCE 700 1- Seki Shuhji Division of Basic Traumatology, National Defense Medical College Research Institute, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut NATIONALLICENCE 700 1- Hiraide Hoshio Division of Basic Traumatology, National Defense Medical College Research Institute, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan, JP aut NATIONALLICENCE 773 0- World Journal of Surgery Springer-Verlag 24/1(2000-01-01), 10-16 0364-2313 24:1<10 2000 24 268 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer